Lee, Wonwoong Um, Jinhee Hwang, Boram Lee, Yong Chan Chung, Bong Chul Hong, Jongki 2024-01-19T18:02:42Z 2024-01-19T18:02:42Z 2021-09-05 2020-03 0960-0760 https://pubs.kist.re.kr/handle/201004/118913 Bile acid (BA) imbalance may be directly associated with gastric cancer and indirectly influence stomach carcinogenesis via overexpression of histidine decarboxylase (HDC), which converts histidine (His) into histamine (HIST). Moreover, the progression of gastric cancer, could change the gut microbiome, including bacteria spp. that produce secondary BAs. Gastric juice has various metabolites that could indicate gastric cancer-related stomach conditions. Therefore, profiling of HIST, His, and BM in gastric juice is crucial for understanding the etiological mechanisms of gastric cancer. We used a profiling method to simultaneously determine targeted metabolites in gastric juice using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We successfully analyzed 70 human gastric juice samples from patients with chronic superficial gastritis (CSG, n = 20), intestinal metaplasia (IM, n = 12), and gastric cancer (n = 38). Furthermore, we investigated the relevance between BA metabolism and gastric cancer. There were statistical differences in the metabolism of cholic acid (CA) into deoxycholic acid (DCA) based on the progression of CSG into IM and gastric cancer. Hence, the progression of gastric cancer might be related to the alterations in gut microbiome composition. We provide insight into the etiological mechanisms of the progression of gastric cancer and biomarkers to diagnose and treat gastric cancer. English PERGAMON-ELSEVIER SCIENCE LTD BENZOYL CHLORIDE DERIVATIZATION MASS-SPECTROMETRY POTENTIAL BIOMARKERS METABOLOME SERUM FLUID COLON Assessing the progression of gastric cancer via profiling of histamine, histidine, and bile acids in gastric juice using LC-MS/MS Article 10.1016/j.jsbmb.2019.105539 1 JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.197 JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 197 scie scopus 000518664800009 2-s2.0-85075164612 Biochemistry & Molecular Biology Endocrinology & Metabolism Biochemistry & Molecular Biology Endocrinology & Metabolism Article BENZOYL CHLORIDE DERIVATIZATION MASS-SPECTROMETRY POTENTIAL BIOMARKERS METABOLOME SERUM FLUID COLON Bile acids Histidine decarboxylase LC-MS/MS Gut microbiome Gastric juice Gastric cancer