Choi, Seungbum Park, Yae Eun Cheon, Eun Jeong Kim, Kyeong Yeon Kim, Miso Ann, Soo-jin Noh, Hye-Min Lee, Jaeho Lee, Chan Joo Lee, Seung-Taek Lee, Cheolju Lee, Ji Eun Lee, Sang-Hak 2024-01-19T18:02:58Z 2024-01-19T18:02:58Z 2021-09-04 2020-03 1738-5520 https://pubs.kist.re.kr/handle/201004/118928 Background and Objectives: Recent studies have examined the structure-function relationship of high-density lipoprotein (HDL). This study aimed to identify and rank HDL-associated proteins involved in several biological function of HDL. Methods: HDLs isolated from 48 participants were analyzed. Cholesterol efflux capacity, effect of HDL on nitric oxide production, and vascular cell adhesion molecule-1 expression were assessed. The relative abundance of identified proteins in the highest vs. lowest quartile was expressed using the normalized spectral abundance factor ratio. Results: After adjustment by multiple testing, six proteins, thyroxine-binding globulin, alpha-1B-glycoprotein, plasma serine protease inhibitor, vitronectin, angiotensinogen, and serum amyloid A-4, were more abundant (relative abundance ratio >= 2) in HDLs with the highest cholesterol efflux capacity. In contrast, three proteins, complement C4-A, alpha-2-macroglobulin, and immunoglobulin mu chain C region, were less abundant (relative abundance ratio <0.5). In terms of nitric oxide production and vascular cell adhesion molecule-1 expression, no proteins showed abundance ratios >= 2 or <0.5 after adjustment. Proteins correlated with the functional parameters of HDL belonged to diverse biological categories. Conclusions: In summary, this study ranked proteins showing higher or lower abundance in HDLs with high functional capacities and newly identified multiple proteins linked to cholesterol efflux capacity. English KOREAN SOC CARDIOLOGY EPITHELIUM-DERIVED FACTOR GROWTH-FACTOR-I BINDING PROTEIN-3 ANTIINFLAMMATORY PROPERTIES HDL ATHEROSCLEROSIS VITRONECTIN SUPPRESSION ACTIVATION PATHWAYS Novel Associations between Related Proteins and Cellular Effects of High-Density Lipoprotein Article 10.4070/kcj.2019.0195 1 KOREAN CIRCULATION JOURNAL, v.50, no.3, pp.236 - 247 KOREAN CIRCULATION JOURNAL 50 3 236 247 scie scopus kci ART002558874 000517831100005 2-s2.0-85081545168 Cardiac & Cardiovascular Systems Cardiovascular System & Cardiology Article EPITHELIUM-DERIVED FACTOR GROWTH-FACTOR-I BINDING PROTEIN-3 ANTIINFLAMMATORY PROPERTIES HDL ATHEROSCLEROSIS VITRONECTIN SUPPRESSION ACTIVATION PATHWAYS Atherosclerosis Cardiovascular diseases Proteomics Lipoproteins Immunity