Kim, Hye Ri Song, Min Yong Kim, Byoung Chan 2024-01-19T18:03:24Z 2024-01-19T18:03:24Z 2021-09-05 2020-02-15 0003-2697 https://pubs.kist.re.kr/handle/201004/118952 Usually, isolation of bacteria-specific aptamers by SELEX is a time-consuming process due to the required repeated rounds of binding, separation, and amplification of the probes to target bacteria. Here, we show that it is possible to isolate bacteria-specific DNA aptamers omitting the repeated rounds of binding incubation, separation, and amplification that are indispensable for SELEX. The serial removal of unbound DNAs to the bacterial cells from an initial mixture of bacteria and DNA libraries through serial centrifugation, one-time separation, and further one-time amplification of DNA bound to the target bacterial cells applied in this non-SELEX-based method allows successful aptamer isolation. English ACADEMIC PRESS INC ELSEVIER SCIENCE EXPONENTIAL ENRICHMENT SYSTEMATIC EVOLUTION DNA APTAMERS SELECTION LIGANDS PATHOGENS MOLECULES Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method Article 10.1016/j.ab.2019.113542 1 ANALYTICAL BIOCHEMISTRY, v.591 ANALYTICAL BIOCHEMISTRY 591 scie scopus 000518181200007 2-s2.0-85076558668 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article EXPONENTIAL ENRICHMENT SYSTEMATIC EVOLUTION DNA APTAMERS SELECTION LIGANDS PATHOGENS MOLECULES Centrifugation-based partitioning Rapid isolation Bacteria-specific aptamer Non-SELEX-Based method Escherichia coli