Choi, Eunshil Lim, Dong-Kwon Kim, Sehoon 2024-01-19T18:03:30Z 2024-01-19T18:03:30Z 2021-09-04 2020-02-15 0021-9797 https://pubs.kist.re.kr/handle/201004/118957 Delivery of apoptosis-associated proteins is an attractive approach to treat cancer, but their large molecular sizes and membrane-impermeability require the use of a suitable delivery carrier. As a versatile drug carrier, mesoporous silica nanoparticles (MSNs) have been utilized to transport a variety of therapeutic molecules. However, the use of MSNs for protein delivery has been limited because their conventionally obtainable pore size (ca. 2-3 nm in diameter) is too small to load large-sized biomolecular cargos. In this article, we present surface erosion of MSNs by hydrolytic degradation as a new strategy to obtain a mesoporous colloidal carrier for effective delivery of a bulky apoptosis-inducible protein, cytochrome c (CYT). A series of physicochemical properties of particles were analyzed before and after the hydrolytic surface erosion of pristine small-pored MSNs and the subsequent CYT loading. The results showed that hydrolytic degradation of MSNs imparts beneficial structural features for CYT loading and release, i.e., enlarged pores (up to similar to 10 nm in diameter) and roughened surface texture, leading to significantly enhanced intracellular delivery of CYT over conventional small-pored MSNs. The present results may offer a useful insight into silica degradability for tuning the internal/external surface characteristics of MSN-based colloidal particles to open a wide range of biomedical applications. (C) 2019 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE IN-VITRO DEGRADATION BEHAVIOR DRUG-DELIVERY RELEASE SPHERES FORM Hydrolytic surface erosion of mesoporous silica nanoparticles for efficient intracellular delivery of cytochrome c Article 10.1016/j.jcis.2019.10.100 1 JOURNAL OF COLLOID AND INTERFACE SCIENCE, v.560, pp.416 - 425 JOURNAL OF COLLOID AND INTERFACE SCIENCE 560 416 425 scie scopus 000504130200044 2-s2.0-85074163363 Chemistry, Physical Chemistry Article IN-VITRO DEGRADATION BEHAVIOR DRUG-DELIVERY RELEASE SPHERES FORM Mesoporous silica Degradation Large pore Rough surface Cytochrome c Drug delivery