Eom, Kyu Shik Lee, Yi Jae Seo, Hye Won Kang, Ji Yoon Shim, Joon Sub Lee, Soo Hyun 2024-01-19T18:03:33Z 2024-01-19T18:03:33Z 2021-09-05 2020-02-07 0003-2654 https://pubs.kist.re.kr/handle/201004/118960 An excessive cholesterol level can lead to cardiovascular diseases, such as stroke, hypertension, and myocardial infarction. A non-invasive, painless method of determining the cholesterol level in blood would improve the user's convenience. To provide rapid and accurate determination of cholesterol, we have developed a simple, disposable, enzyme-based electrochemical biosensor that can detect salivary cholesterol. It is possible to detect low concentrations of cholesterol in saliva using the optimized vertical structure of the platinum nano-cluster (Pt-NC) and the immobilization of a proper volume of an enzyme. The biosensor exhibited a linear range from 2 to 486 mu M, the limit of detection was about 2 mu M, and the sensitivity of the sensor was calculated to be 132 mu A mM(-1) cm(-2). It also showed good specificity for ascorbic acid, uric acid, dopamine, glucose, and lactate. In a test with an actual sample, the performance of the biosensor was confirmed by measuring total cholesterol in the saliva of a patient with hyperlipidemia. The cholesterol levels measured in the saliva of three patients with hyperlipidemia were 520, 460, and 290 mu M. Therefore, the Pt-NC based enzyme sensor is a promising candidate for the detection of cholesterol in human saliva. English Royal Society of Chemistry BIOSENSOR OXIDASE NANOPARTICLES POLYMER ACID Sensitive and non-invasive cholesterol determination in saliva via optimization of enzyme loading and platinum nano-cluster composition Article 10.1039/c9an01679a 1 Analyst, v.145, no.3, pp.908 - 916 Analyst 145 3 908 916 scie scopus 000528803100018 2-s2.0-85079020950 Chemistry, Analytical Chemistry Article BIOSENSOR OXIDASE NANOPARTICLES POLYMER ACID cholesterol sesnor saliva Platinum nano-cluster enzyme blood optimization