Seok, Ji Min Rajangam, Thanavel Jeong, Jae Eun Cheong, Sinyoung Joo, Sang Min Oh, Seung Ja Shin, Heungsoo Kim, Sang-Heon Park, Su A. 2024-01-19T18:03:34Z 2024-01-19T18:03:34Z 2021-09-05 2020-02-07 2050-7518 https://pubs.kist.re.kr/handle/201004/118962 Scaffold porosity has played a key role in bone tissue engineering aimed at effective tissue regeneration, by promoting cell attachment, proliferation, and osteogenic differentiation for new bone formation. Three-dimensional plotting systems (3DPSs) have been widely used to introduce porosity to the scaffold; however, introducing certain features in the scaffold strands that improve bone tissue regeneration remains a challenge. In this work, we fabricated bone tissue scaffolds with macro- and microporous structural features using a 3DPS and non-solvent-induced phase separation method. This approach allowed both macro- and micropores to be created in the scaffold strands. The surface morphology and mechanical and degradation properties of the perforated scaffolds were characterized carefully. Human marrow stromal cells were cultured on the scaffolds and then analyzed in vitro to assess scaffold bio-function. The highly porous scaffold exhibited mechanical properties similar to those of cancellous bone. Cell attachment, proliferation, and differentiation were significantly higher in porous scaffold compared to its nonporous counterpart. These results suggest that highly porous scaffolds have tremendous potential as a bone tissue regeneration platform. English ROYAL SOC CHEMISTRY MECHANICAL-PROPERTIES REGENERATION PCL POLYCAPROLACTONE DIFFERENTIATION MACROPOROSITY POROSITY DESIGN Fabrication of 3D plotted scaffold with microporous strands for bone tissue engineering Article 10.1039/c9tb02360g 1 JOURNAL OF MATERIALS CHEMISTRY B, v.8, no.5, pp.951 - 960 JOURNAL OF MATERIALS CHEMISTRY B 8 5 951 960 scie scopus 000528811300008 2-s2.0-85079020112 Materials Science, Biomaterials Materials Science Article MECHANICAL-PROPERTIES REGENERATION PCL POLYCAPROLACTONE DIFFERENTIATION MACROPOROSITY POROSITY DESIGN