Kim, Jungyeon Kim, Joongsuk Um, Youngsoon Kim, Kyoung Heon 2024-01-19T18:04:11Z 2024-01-19T18:04:11Z 2021-09-05 2020-02 1359-5113 https://pubs.kist.re.kr/handle/201004/118996 Clostridium carboxidivorans ferments CO, CO2, and H-2 via the Wood-Ljungdahl pathway. CO, CO2, and H-2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation. English Elsevier Applied Science Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide Article 10.1016/j.procbio.2019.10.012 1 Process Biochemistry, v.89, pp.20 - 28 Process Biochemistry 89 20 28 N scie scopus 000517856100003 2-s2.0-85075423815 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Engineering, Chemical Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Engineering Article SYNTHESIS GAS FERMENTATION METABOLOMICS BUTANOL SYNGAS BIOFUELS Metabolomics Extraction solvent Optimization Clostridium carboxidivorans Carbon monoxide