Peng, Lei Ahn, Hong Ryul Jung, Sang Hoon Choi, Yongsoo 2024-01-19T18:04:39Z 2024-01-19T18:04:39Z 2021-09-04 2020-02 0253-2964 https://pubs.kist.re.kr/handle/201004/119025 Although recent pharmacological studies have demonstrated the beneficial effects of the ethanol extract of Diospyros kaki (EEDK) against eye diseases in vitro and in vivo, they could not conclude whether two strong antioxidants, quercetin and kaempferol, in the EEDK were bioavailable in eye tissue after oral administration. A method based on ultra-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-MS/MS) was herein developed for the highly selective and sensitive quantification of quercetin and kaempferol in rat eye tissue. The method was applied to investigate the distribution of quercetin and kaempferol in rat eyes after oral administration of EEDK at 0, 5, and 35 min after it was successfully validated for specificity, linearity, a low limit of quantification, precision, accuracy, extraction recovery, and matrix effect. The distribution of quercetin and kaempferol in rat eyes at 35 min after oral administration of the EEDK was 0.0099% and 0.0041%, respectively. These results will be useful for the precise determination of optimized in vivo EEDK dose and time for the effective prevention and treatment of eye diseases. English 대한화학회 Quantification and Distribution Evaluation of Quercetin and Kaempferol in Rat Eyes after Oral Administration of EEDK Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Article 10.1002/bkcs.11926 1 Bulletin of the Korean Chemical Society, v.41, no.2, pp.117 - 122 Bulletin of the Korean Chemical Society 41 2 117 122 N scie scopus kci ART002557894 000503204000001 2-s2.0-85076733426 Chemistry, Multidisciplinary Chemistry Article DIOSPYROS-KAKI PERSIMMON LEAVES EXTRACT TISSUE Tandem mass spectrometry Ethanol extract of Diospyros kaki Quercetin and kaempferol Distribution Rat eyes