Cho, Yu Sung Kim, Hye Ri Ko, Hyun Sik Jeong, Sang Bin Kim, Byoung Chan Jung, Jae Hee 2024-01-19T18:04:47Z 2024-01-19T18:04:47Z 2021-09-04 2020-02 2379-3694 https://pubs.kist.re.kr/handle/201004/119032 Real-time on-site monitoring of bioaerosols in an air environment is important for preventing various adverse health effects including respiratory diseases and allergies caused by bioaerosols. Here, we report the development of an on-site automated bioaerosol-monitoring system (ABMS) using integrated units including a wet-cyclone bioaerosol sampler, a thermal-lysis unit for extracting adenosine triphosphate (ATP), an ATP-detection unit based on the immobilization of luciferase/luciferin for bioluminescence reactions, and a photomultiplier tube-based detector. The performance of the bioaerosol detection system was verified using Escherichia coli (E. coli) as a model source. Each unit was optimized to process similar to 9.6 x 10(5) times the concentrated ratio of collected bioaerosol samples, using a 3 min lysis time to extract ATP, and has a detection limit of similar to 375 colony-forming units (CFUs)/mL with more than 30 days of stability for the immobilizedluciferase/luciferin detection unit supported by a glass-fiber conjugation pad. After the integration of all units, the ABMS achieved E. coli bioaerosol monitoring with continuous detection at 5 min intervals and a minimum detection limit of similar to 130 CFU/m(air)(3). Furthermore, the rapid responsivity and stable operation performance of the ABMS under test-bed conditions and during a field test demonstrated that the ABMS is capable of continuously monitoring bioaerosols in real-time with high sensitivity. The monitoring system developed here with immobilization strategies for bioluminescence reactions triggered by ATP extracted from collected bioaerosol samples using a simple heat-lysis method may help establish sustainable platforms to obtain stable signals for the real-time detection of bioaerosols on-site. English AMER CHEMICAL SOC AIRBORNE MICROORGANISMS BACTERIA ATP AIR INDOOR PCR ENUMERATION EXPOSURE HEALTH ASSAY Continuous Surveillance of Bioaerosols On-Site Using an Automated Bioaerosol-Monitoring System Article 10.1021/acssensors.9b02001 1 ACS Sensors, v.5, no.2, pp.395 - 403 ACS Sensors 5 2 395 403 scie scopus 000517856700015 2-s2.0-85078992646 Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Chemistry Science & Technology - Other Topics Article AIRBORNE MICROORGANISMS BACTERIA ATP AIR INDOOR PCR ENUMERATION EXPOSURE HEALTH ASSAY indoor air quality automated bioaerosol-monitoring system bioaerosols continuous surveillance wet-cyclone luciferase/luciferin immobilization