Rigual, Victoria Papa, Gabriella Rodriguez, Alberto Wehrs, Maren Kim, Kwang Ho Oliet, Mercedes Alonso, M. Virginia Gladden, John M. Mukhopadhyay, Aindrila Simmons, Blake A. Singh, Seema 2024-01-19T18:30:27Z 2024-01-19T18:30:27Z 2021-09-05 2020-01-20 2168-0485 https://pubs.kist.re.kr/handle/201004/119079 Woody biomass feedstocks pose challenges in their use as sustainable carbon sources due to their recalcitrance. One-pot ionic liquid-based processes are a promising bioproduction strategy to reduce water consumption (removing the washing of biomass), reduce costs, and minimize the presence of inhibitory compounds. We evaluated the one-pot ionic liquid pretreatment + saccharification configuration as a scheme for the deconstruction and conversion of two different woody feedstocks, eucalyptus, and pine. A comparative study of protic ionic liquids 2-hydroxylethylammonium acetate-based ionic liquids, bis-2-hydroxyethylammonium acetate, and cholinium lysinate for pretreatment of eucalyptus and pine was conducted. The impact of ionic liquids at 11% w/w on the saccharification and toxicity of hydrolysates containing the ionic liquid for downstream use for microbial cultivation was evaluated. Protic ionic liquids were more effective in eucalyptus than in pine pretreatment and less toxic than cholinium lysinate at concentrations <= 10% w/w. 2-hydroxylethylammonium acetate yielded the highest digestibility, of up to 75% in eucalyptus. As a proof of concept of an integrated feedstocks-to-biofuels process, we tested the growth of the fungal strain Rhodosporidium toruloides in hydrolysates with low amounts of ionic liquids and phenolic compounds. The proof of concept demonstrated the ability of Rhodosporidium toruloides to grow in the presence of 2-hydroxyethylammonium acetate (5% w/w), the importance to adjust the pH after saccharification, and the ability to consume lignin monomers. This work offers a valid starting point for further studies aimed at increasing cellulose digestibility in a one-pot configuration in the presence of protic ionic liquids. English American Chemical Society ENZYMATIC-HYDROLYSIS RICE STRAW LIGNOCELLULOSIC BIOMASS LIGNIN CONTENT CELLULOSE CHOLINIUM DEPOLYMERIZATION RECALCITRANCE DEGRADATION EUCALYPTUS Evaluating Protic Ionic Liquid for Woody Biomass One-Pot Pretreatment plus Saccharification, Followed by Rhodosporidium toruloides Cultivation Article 10.1021/acssuschemeng.9b04451 1 ACS Sustainable Chemistry & Engineering, v.8, no.2, pp.782 - 791 ACS Sustainable Chemistry & Engineering 8 2 782 791 scie scopus 000509432200007 2-s2.0-85078661978 Chemistry, Multidisciplinary Green & Sustainable Science & Technology Engineering, Chemical Chemistry Science & Technology - Other Topics Engineering Article ENZYMATIC-HYDROLYSIS RICE STRAW LIGNOCELLULOSIC BIOMASS LIGNIN CONTENT CELLULOSE CHOLINIUM DEPOLYMERIZATION RECALCITRANCE DEGRADATION EUCALYPTUS One-pot Ionic liquids Eucalyptus Pine Rhodosporidium toruloides Enzymatic hydrolysis