Ahn, Sae Ryun An, Ji Hyun Lee, Seung Hwan Song, Hyun Seok Jang, Jyongsik Park, Tai Hyun 2024-01-19T18:30:33Z 2024-01-19T18:30:33Z 2021-09-05 2020-01-15 2045-2322 https://pubs.kist.re.kr/handle/201004/119085 Hormones within very low levels regulate and control the activity of specific cells and organs of the human body. Hormone imbalance can cause many diseases. Therefore, hormone detection tools have been developed, particularly over the last decade. Peptide hormones have a short half-life, so it is important to detect them within a short time. In this study, we report two types of peptide hormone sensors using human hormone receptor-carrying nanovesicles and graphene field-effect transistors (FETs). Parathyroid hormone (PTH) and glucagon (GCG) are peptide hormones present in human blood that act as ligands to G protein-coupled receptors (GPCRs). In this paper, the parathyroid hormone receptor (PTHR) and the glucagon receptor (GCGR) were expressed in human embryonic kidney-293 (HEK-293) cells, and were constructed as nanovesicles carrying the respective receptors. They were then immobilized onto graphene-based FETs. The two hormone sensors developed were able to detect each target hormone with high sensitivity (ca. 100 fM of PTH and 1 pM of GCG). Also, the sensors accurately recognized target hormones among different types of peptide hormones. In the development of hormone detection tools, this approach, using human hormone receptor-carrying nanovesicles and graphene FETs, offers the possibility of detecting very low concentrations of hormones in real-time. English NATURE PUBLISHING GROUP FIELD-EFFECT TRANSISTOR HUMAN TASTE RECEPTOR PARATHYROID-HORMONE BIOELECTRONIC TONGUE HIGH-SENSITIVITY NOSE RADIOIMMUNOASSAY APTASENSOR MIMICKING PLATFORM Peptide hormone sensors using human hormone receptor-carrying nanovesicles and graphene FETs Article 10.1038/s41598-019-57339-1 1 SCIENTIFIC REPORTS, v.10, no.1 SCIENTIFIC REPORTS 10 1 scie scopus 000551445800004 2-s2.0-85077941840 Multidisciplinary Sciences Science & Technology - Other Topics Article FIELD-EFFECT TRANSISTOR HUMAN TASTE RECEPTOR PARATHYROID-HORMONE BIOELECTRONIC TONGUE HIGH-SENSITIVITY NOSE RADIOIMMUNOASSAY APTASENSOR MIMICKING PLATFORM