Lee, Junwoo Kim, Hyerin Heo, Youhee Yoo, Yong Kyoung Han, Sung Il Kim, Cheonjung Hur, Don Kim, Hyungsuk Kang, Ji Yoon Lee, Jeong Hoon 2024-01-19T18:30:46Z 2024-01-19T18:30:46Z 2021-09-05 2020-01-07 0003-2654 https://pubs.kist.re.kr/handle/201004/119098 EVs/exosomes are considered as the next generation of biomarkers, including for liquid biopsies. Consequently, the quantification of EVs/exosomes is crucial for facilitating EV/exosome research and applications. Paper-based enzyme-linked immunosorbent assay (p-ELISA) is a portable diagnostic system with low cost that is simple and easy to use; however, it shows low sensitivity and linearity. In this study, we develop p-ELISA for targeting EVs/exosomes by using streptavidin agarose resin-based immobilization (SARBI). This method reduces assay preparation times, provides strong binding, and retains good sensitivity and linearity. The time required for the total assay, including preparation steps and surface immobilization, was shortened to similar to 2 h. We evaluated SARBI p-ELISA systems with/without CD63 capture Ab and then with fetal bovine serum (FBS) and EVs/exosome-depleted fetal bovine serum (dFBS). The results provide evidence supporting the selective capture ability of SARBI p-ELISA. We obtain semiquantitative p-ELISA results using an exosome standard (ES) and human serum (HS), with R-2 values of 0.95 and 0.92, respectively. English Royal Society of Chemistry LINKED-IMMUNOSORBENT-ASSAY EXTRACELLULAR VESICLES EXOSOMES ANTIBODY BLOOD Enhanced paper-based ELISA for simultaneous EVs/exosome isolation and detection using streptavidin agarose-based immobilization Article 10.1039/c9an01140d 1 Analyst, v.145, no.1, pp.157 - 164 Analyst 145 1 157 164 scie scopus 000502788600014 2-s2.0-85076583799 Chemistry, Analytical Chemistry Article LINKED-IMMUNOSORBENT-ASSAY EXTRACELLULAR VESICLES EXOSOMES ANTIBODY BLOOD