Kim, Hye Jin Choi, Woongsun Kim, Jinsik Choi, Jungkyu Choi, Nakwon Hwang, Kyo Seon 2024-01-19T18:30:50Z 2024-01-19T18:30:50Z 2021-09-05 2020-01-01 0925-4005 https://pubs.kist.re.kr/handle/201004/119102 This study focused on fabricating a target-specific electrical biosensor with high sensitivity and dynamic range by embedding a hydrogel, that incorporated a much larger number of antibodies in the reaction region of the sensor, allowing all biomolecules except the target molecules to be filtered off. The biosensor is comprised of a three-dimensional interdigitated microelectrode (3D IMEs) with much higher aspect ratios than conventional IMEs and a hydrogel with a 3D mesh structure. The hydrogel increases the number of antibodies in the reaction region of the 3D IMEs by approximately 1,000-fold and enhances the impedance change induced by the specific binding of biomolecules. The hydrogel density is altered according to the target biomolecules. The impedance change by A beta(42 )binding was maximized to approximately 12.135 +/- 0.903% in the 3D IMEs with a 'dense' hydrogel. The change by prostate-specific antigen (PSA) binding was maximized in the 3D IMEs with a 'loose' hydrogel. Thus, the dynamic range and sensitivity for detecting A beta(42) were enhanced by a maximum of two orders and by approximately 2.58-fold compared to planar IMEs. These results demonstrate the high performance of the hydrogel-embedded 3D IMEs and its applicability for biological fluid-based diagnosis. English ELSEVIER SCIENCE SA FILTRATION PLASMA Highly sensitive three-dimensional interdigitated microelectrode biosensors embedded with porosity tunable hydrogel for detecting proteins Article 10.1016/j.snb.2019.127190 1 SENSORS AND ACTUATORS B-CHEMICAL, v.302 SENSORS AND ACTUATORS B-CHEMICAL 302 scie scopus 000489830200022 2-s2.0-85072670535 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article FILTRATION PLASMA Porosity tunable hydrogel Three-dimensional interdigitated microelectrodes High sensitivity and selectivity Amyloid beta