Ko, Young Ji Lee, Jae-Won Yang, Eun-Jeong Jang, Nayoon Park, Jooho Jeon, Yoon Kyung Yu, Je-Wook Cho, Nam-Hyuk Kim, Hye-Sun Kwon, Ick Chan 2024-01-19T18:31:19Z 2024-01-19T18:31:19Z 2021-09-05 2020-01 0142-9612 https://pubs.kist.re.kr/handle/201004/119127 Inflammasome plays a critical role in diverse inflammatory disorders, including cancers and Alzheimer's disease. It is induced by various pathogenic insults and activates caspase-1, a hallmark executor of inflammasome. Here, we developed an activatable fluorescence probe for visualization of active caspase-1. This caspase-1 probe is biocompatible, efficiently delivered into cells and tissues, and specifically emits fluorescence upon caspase-1 activation as assessed in in vitro and in vivo models of inflammatory conditions. We demonstrated efficient in vivo imaging of caspase-1 activation in early stages of various inflammatory conditions of mice models, including endotoxin shock, inflammatory bowel disorder, transplanted cancer, and Alzheimer's disease. Notably, the caspase-1 probe enables detection of neuroinflammation in vivo two months earlier than cognitive impairments occur in Alzheimer's disease model. We detected significant fluorescence emitted from inflamed sites, as well as their draining lymph nodes, by macroscopic imaging analysis within 30 min after systemic injection of the probe. This novel synthetic probe could be applied for efficient and rapid detection of caspase-1 activity in a spatiotemporal way by non-invasive imaging. English ELSEVIER SCI LTD INFLAMMASOMES MECHANISM ASC SPECKS CELL DISEASE MOUSE DYNAMICS PLATFORM REVEALS INNATE ROLES Non-invasive in vivo imaging of caspase-1 activation enables rapid and spatiotemporal detection of acute and chronic inflammatory disorders Article 10.1016/j.biomaterials.2019.119543 1 BIOMATERIALS, v.226 BIOMATERIALS 226 scie scopus 000498330900008 2-s2.0-85073570990 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article INFLAMMASOMES MECHANISM ASC SPECKS CELL DISEASE MOUSE DYNAMICS PLATFORM REVEALS INNATE ROLES Inflammasome Caspase-1 Activatable fluorescence probe Inflammation Cancer Alzheimer&apos s disease