Hoang Nguyen Tran, Phuong Ko, Ja Kyong Gong, Gyeongtaek Um, Youngsoon Lee, Sun-Mi 2024-01-19T18:31:38Z 2024-01-19T18:31:38Z 2021-09-05 2020-01 https://pubs.kist.re.kr/handle/201004/119145 Background Lignocellulosic biorefinery offers economical and sustainable production of fuels and chemicals. Saccharomyces cerevisiae, a promising industrial host for biorefinery, has been intensively developed to expand its product profile. However, the sequential and slow conversion of xylose into target products remains one of the main challenges for realizing efficient industrial lignocellulosic biorefinery. Results In this study, we developed a powerful mixed-sugar co-fermenting strain of S. cerevisiae, XUSEA, with improved xylose conversion capacity during simultaneous glucose/xylose co-fermentation. To reinforce xylose catabolism, the overexpression target in the pentose phosphate pathway was selected using a DNA assembler method and overexpressed increasing xylose consumption and ethanol production by twofold. The performance of the newly engineered strain with improved xylose catabolism was further boosted by elevating fermentation temperature and thus significantly reduced the co-fermentation time by half. Through combined efforts of reinforcing the pathway of xylose catabolism and elevating the fermentation temperature, XUSEA achieved simultaneous co-fermentation of lignocellulosic hydrolysates, composed of 39.6 g L-1 glucose and 23.1 g L-1 xylose, within 24 h producing 30.1 g L-1 ethanol with a yield of 0.48 g g(-1). Conclusions Owing to its superior co-fermentation performance and ability for further engineering, XUSEA has potential as a platform in a lignocellulosic biorefinery toward realizing a more economical and sustainable process for large-scale bioethanol production. English BioMed Central Improved simultaneous co-fermentation of glucose and xylose by Saccharomyces cerevisiae for efficient lignocellulosic biorefinery Article 10.1186/s13068-019-1641-2 1 Biotechnology for Biofuels, v.13, no.1 Biotechnology for Biofuels 13 1 Y scie scopus 000513589200001 2-s2.0-85079103589 Biotechnology & Applied Microbiology Energy & Fuels Biotechnology & Applied Microbiology Energy & Fuels Article ETHANOL-PRODUCTION YEAST ISOMERASE TRANSPORTERS STRAIN ISOMERIZATION CONSTRUCTION TEMPERATURE BIOETHANOL EVOLUTION Lignocellulosic biorefinery Efficient co-fermentation Saccharomyces cerevisiae Xylose isomerase Bioethanol