Lee, Na-Eun Park, Sang-Deuk Hwang, Hongik Choi, Sun-Hye Lee, Ra Mi Nam, Sung Min Choi, Jong Hee Rhim, Hyewhon Cho, Ik-Hyun Kim, Hyoung-Chun Hwang, Sung-Hee Nah, Seung-Yeol 2024-01-19T18:32:00Z 2024-01-19T18:32:00Z 2021-09-04 2020-01 1226-8453 https://pubs.kist.re.kr/handle/201004/119166 Background: Ginseng has been widely used as a health-promoting tonic. Gintonin present in ginseng acts as a lysophosphatidic acid (LPA) receptor ligand that activates six LPA receptor subtypes. The LPA6 subtype plays a key role in normal hair growth, and mutations in the LPA6 receptor impair normal human hair growth. Currently, human hair loss and alopecia are concerning issues that affect peoples' social and day-to-day lives. Objective: We investigated the in vitro and in vivo effects of a gintonin-enriched fraction (GEF) on mouse hair growth. Methods: Human hair follicle dermal papilla cells (HFDPCs) and six-week-old male C57BL/6 mice were used. The mice were divided into the four groups: control, 1% minoxidil, 0.75% GEF, and 1.5% GEF. The dorsal hair was removed to synchronize the telogen phase. Each group was treated topically, once a day, for 15 days. We analyzed hair growth activity and histological changes. Results: GEF induced transient [Ca2+](i), which stimulated HFDPC proliferation and caused 5-bromo-2'-deoxyuridine (BrdU) incorporation in a concentration-dependent manner. GEF-mediated HFDPC proliferation was blocked by the LPA receptor antagonist and Ca2+ chelator. HFDPC treatment with GEF stimulated vascular endothelial growth factor release. Topical application of GEF and minoxidil promoted hair growth in a dose-dependent manner. Histological analysis showed that GEF and minoxidil increased the number of hair follicles and hair weight. Conclusion: Topical application of GEF promotes mouse hair growth through HFDPC proliferation. GEF could be one of the main components of ginseng that promote hair growth and could be used to treat human alopecia. (C) 2019 The Korean Society of Ginseng. Publishing services by Elsevier B.V. English 고려인삼학회 Effects of a gintonin-enriched fraction on hair growth: an in vitro and in vivo study Article 10.1016/j.jgr.2019.05.013 1 Journal of Ginseng Research, v.44, no.1, pp.168 - 177 Journal of Ginseng Research 44 1 168 177 Y scie scopus kci ART002550369 000505047900020 2-s2.0-85068081880 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article PROTEIN-COUPLED RECEPTOR ANDROGENETIC ALOPECIA CELL-PROLIFERATION PHOSPHATIDIC-ACID MOUSE MODEL GINSENG ACTIVATION MECHANISMS EXPRESSION STRESS Ginseng Gintonin-enriched fraction Hair growth Human hair follicle dermal papilla cells Mouse