Lee, Rami Lee, Na-Eun Hwang, Hongik Rhim, Hyewhon Cho, Ik-Hyun Nah, Seung-Yeol 2024-01-19T18:33:54Z 2024-01-19T18:33:54Z 2021-09-05 2019-12 1420-3049 https://pubs.kist.re.kr/handle/201004/119279 Gintonin is a newly discovered component of ginseng and acts as a ligand for G protein-coupled lysophosphatidic acid (LPA) receptors. It is currently unclear whether gintonin has skin-related effects. Here, we examined the effects of a gintonin-enriched fraction (GEF) on [Ca2+](i) transient induction in human dermal fibroblasts (HDFs). We found that GEF treatment transiently induced [Ca2+](i) in a dose-dependent manner. GEF also increased cell viability and proliferation, which could be blocked by Ki16425, an LPA1/3 receptor antagonist, or 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), a calcium chelator. We further found that GEF stimulated hyaluronic acid (HA) release from HDFs in a dose- and time-dependent manner, which could be attenuated by Ki16425, U73122, a phospholipase C inhibitor, 2-Aminoethoxydiphenyl borate (2-APB), an IP3 receptor antagonist, and BAPTA-AM. Moreover, we found that GEF increased HA synthase 1 (HAS1) expression in a time-dependent manner. We also found that GEF stimulates collagen release and the expression of collagen 1, 3, and 7 synthases in a time-dependent manner. GEF-mediated collagen synthesis could be blocked by Ki16425, U73122, 2-APB, and BAPTA-AM. GEF treatment also increased the mRNA levels of LPA1-6 receptor subtypes at 8 h and increased the protein levels of LPA1-6 receptor subtypes at 8 h. Overall, these results indicate that the GEF-mediated transient induction of [Ca2+](i) is coupled to HA and collagen release from HDFs via LPA receptor regulations. We can, thus, conclude that GEF might exert a beneficial effect on human skin physiology via LPA receptors. English Multidisciplinary Digital Publishing Institute (MDPI) Ginseng Gintonin Enhances Hyaluronic Acid and Collagen Release from Human Dermal Fibroblasts Through Lysophosphatidic Acid Receptor Interaction Article 10.3390/molecules24244438 1 Molecules, v.24, no.24 Molecules 24 24 Y scie scopus 000507299600016 2-s2.0-85076344387 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article PANAX-GINSENG ENRICHED FRACTION MODEL SKIN PROLIFERATION GINSENOSIDES SAPONINS ginseng gintonin human dermal fibroblast hyaluronic acid collagen human skin