Kim, Junghoon Leem, Juyoung Kim, Hong Nam Kang, Pilgyu Choi, Jonghyun Haque, Md Farhadul Kang, Daeshik Nam, SungWoo 2024-01-19T19:00:20Z 2024-01-19T19:00:20Z 2021-09-05 2019-11-04 2055-7434 https://pubs.kist.re.kr/handle/201004/119340 Graphene, owing to its inherent chemical inertness, biocompatibility, and mechanical flexibility, has great potential in guiding cell behaviors such as adhesion and differentiation. However, due to the two-dimensional (2D) nature of graphene, the microfabrication of graphene into micro/nanoscale patterns has been widely adopted for guiding cellular assembly. In this study, we report crumpled graphene, i.e., monolithically defined graphene with a nanoscale wavy surface texture, as a tissue engineering platform that can efficiently promote aligned C2C12 mouse myoblast cell differentiation. We imparted out-of-plane, nanoscale crumpled morphologies to flat graphene via compressive strain-induced deformation. When C2C12 mouse myoblast cells were seeded on the uniaxially crumpled graphene, not only were the alignment and elongation promoted at a single-cell level but also the differentiation and maturation of myotubes were enhanced compared to that on flat graphene. These results demonstrate the utility of the crumpled graphene platform for tissue engineering and regenerative medicine for skeletal muscle tissues. English SPRINGERNATURE CELL ALIGNMENT STEM-CELLS DIFFERENTIATION TISSUE EXPRESSION STIFFNESS DIMENSIONS SURFACES GUIDANCE CUES Uniaxially crumpled graphene as a platform for guided myotube formation Article 10.1038/s41378-019-0098-6 1 MICROSYSTEMS & NANOENGINEERING, v.5 MICROSYSTEMS & NANOENGINEERING 5 scie scopus 000494277800001 2-s2.0-85074367991 Nanoscience & Nanotechnology Instruments & Instrumentation Science & Technology - Other Topics Instruments & Instrumentation Article CELL ALIGNMENT STEM-CELLS DIFFERENTIATION TISSUE EXPRESSION STIFFNESS DIMENSIONS SURFACES GUIDANCE CUES