Si, Yunpei Park, Jeong Won Jung, Sunhee Hwang, Geum-Sook Park, Yae Eun Lee, Ji Eun Lee, Hye Jin 2024-01-19T19:00:25Z 2024-01-19T19:00:25Z 2021-09-04 2019-11-01 0925-4005 https://pubs.kist.re.kr/handle/201004/119345 A highly sensitive and selective voltammetric biosensing platform for the determination of inosine (INO) concentrations and further applications to biological sample solutions was developed. The biosensor configures a layer-by-layer (LbL) self-assembly of the positively charged chitosan (CS) and negatively charged purine nucleoside phosphorylase (PNP) on screen-printed carbon electrode (SPCE); PNP was chosen to improve the selectivity since it is INO selective while CS was used for the enzyme PNP loading. The electrocatalytic reaction of INO and PNP was studied using cyclic voltammetry (CV) and square wave voltammetry (SWV). Under the optimal condition with two-bilayer of the PNP and CS on the modified electrode (PNP CS)(2)-SPCE, a linear dynamic range from 2 to 90 mu M INO with a limit of detection of 0.3 mu M was achieved when using SWV method. Finally, the developed biosensing platform was applied to the detection of INO in both normal human serum sample and myocardial infarction (MI) patient serum sample in addition to INO dietary supplements. The sensing results of normal human and MI patient serum samples were further compared to those obtained from liquid chromatography-mass spectrometry (LC MS) and a commercial inosine assay kit. English ELSEVIER SCIENCE SA HUMAN METABOLOME DATABASE URIC-ACID SALIVARY BIOMARKERS ENZYME ELECTRODE HUMAN PLASMA HYPOXANTHINE ADENOSINE HMDB XANTHINE STRATEGIES Voltammetric layer-by-layer biosensor featuring purine nucleoside phosphorylase and chitosan for inosine in human serum solutions Article 10.1016/j.snb.2019.126840 1 SENSORS AND ACTUATORS B-CHEMICAL, v.298 SENSORS AND ACTUATORS B-CHEMICAL 298 scie scopus 000483419800029 2-s2.0-85069709675 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article HUMAN METABOLOME DATABASE URIC-ACID SALIVARY BIOMARKERS ENZYME ELECTRODE HUMAN PLASMA HYPOXANTHINE ADENOSINE HMDB XANTHINE STRATEGIES Inosine Purine nucleoside phosphorylase Layer-by-layer biosensor Serum Myocardial infarction