Yu, Ji Hyun Lim, Sun Woo Luo, Kang Cui, Sheng Quan, Yi Shin, Yoo Jin Lee, Kyung Eun Kim, Hong Lim Ko, Eun Jeong Chung, Byung Ha Kim, Ju Hwan Chung, Sang J. Yang, Chul Woo 2024-01-19T19:01:02Z 2024-01-19T19:01:02Z 2021-09-05 2019-11 0892-6638 https://pubs.kist.re.kr/handle/201004/119380 The major side effect of tacrolimus (Tac) is nephrotoxicity. We studied whether supplementation of coenzyme Q(10), (CoQ(10)) a potent antioxidant, can reduce Tac-induced nephrotoxicity via improving mitochondrial function. In an in vitro study, CoQ(10) reduced the production of Tac-induced mitochondrial reactive oxygen species and abolished the loss of mitochondrial membrane potential in proximal tubular cell line. Assessment of mitochondrial function revealed that CoQ(10) decreased oxygen consumption and mitochondrial respiration rate increased by Tac, suggesting improvement of mitochondrial function to synthesize ATP with CoQ(10) treatment. The effect of the CoQ(10) in vitro study was observed in an experimental model of chronic Tac-induced nephropathy. CoQ(10) attenuated Tac-induced oxidative stress and was accompanied by function and histologic improvement. On electron microscopy, addition of CoQ(10) increased not only the number but also the volume of mitochondria compared with Tac treatment only. Our data indicate that CoQ(10) improves Tac-induced mitochondrial dysfunction in kidney. Supplementary CoQ(10) treatment may be a promising approach to reduce Tac-induced nephrotoxicity. English Federation of American Societies for Experimental Biology Coenzyme Q(10) alleviates tacrolimus-induced mitochondrial dysfunction in kidney Article 10.1096/fj.201900386RR 1 The FASEB Journal, v.33, no.11, pp.12288 - 12298 The FASEB Journal 33 11 12288 12298 N scie scopus 000507461600054 2-s2.0-85074379489 Biochemistry & Molecular Biology Biology Cell Biology Biochemistry & Molecular Biology Life Sciences & Biomedicine - Other Topics Cell Biology Article CHRONIC CYCLOSPORINE NEPHROPATHY ASCORBIC-ACID PALMITATE OXIDATIVE STRESS FK506 NEPHROTOXICITY EXPERIMENTAL-MODEL BETA-CAROTENE REDUCED FORM VITAMIN-E TROLOX-C ACETYLCYSTEINE 3D reconstruction nephrotoxicity reactive oxygen species