Park, DoYeun Cheng, Jie Park, Jong Bo Shin, Sungchul Lee, Sang-Hoon Hong, Byung Hee Kim, Soo Hyun Hyun, Jinho Yang, Chaoyong 2024-01-19T19:01:13Z 2024-01-19T19:01:13Z 2021-09-05 2019-11 2373-9878 https://pubs.kist.re.kr/handle/201004/119390 An aqueous two-phase system (ATPS) is a water-in-water biphasic system, which is generally formed by two incompatible polymers. Recently, considerable effort has been dedicated to search for new ATPS polymer pairs to further expand ATPS's applications. In this paper, a new ATPS system based on silk fibroin (SF) and alginate is introduced. A phase diagram was established to show the critical concentrations for the formation of an SF/alginate ATPS. The present system is sensitive to pH stimulus and transformed from an ATPS into a single-phasic system as pH increases above similar to 9.5. Circular dichroism, fluorescence emission spectra, hydrodynamic diameter, and zeta-potential data together indicate that the SF chains undergo a dramatic extension as pH is increased, which is the reason underlying the pH-triggered phase transition. As feasible applications of this biphasic system, compartmentalized multiplex immunoassay, controlled encapsulation and release, and hierarchical fiber fabrication were demonstrated using the SF/alginate ATPS. English AMER CHEMICAL SOC ALGINATE FIBERS PURIFICATION MICROFIBERS MECHANISM FIBROIN SIZE pH-Triggered Silk Fibroin/Alginate Structures Fabricated in Aqueous Two-Phase System Article 10.1021/acsbiomaterials.9b01216 1 ACS BIOMATERIALS SCIENCE & ENGINEERING, v.5, no.11, pp.5897 - 5905 ACS BIOMATERIALS SCIENCE & ENGINEERING 5 11 5897 5905 scie scopus 000496344800036 2-s2.0-85073143376 Materials Science, Biomaterials Materials Science Article ALGINATE FIBERS PURIFICATION MICROFIBERS MECHANISM FIBROIN SIZE aqueous two-phase system silk fibroin alginate compartmentalized multiplex immunoassay controlled release biomimicking hierarchical fiber