Whang, Keumrai Chang, Jeehan Jung, Kwangyeong Ko, Hyungduk Lee, Jungchul Choi, Inhee Kang, Taewook 2024-01-19T19:02:07Z 2024-01-19T19:02:07Z 2021-09-05 2019-10-15 0003-2700 https://pubs.kist.re.kr/handle/201004/119443 Detection of small metabolites is essential for monitoring and optimizing biological gas conversion. Currently, such detection is typically done by liquid chromatography with offline sampling. However, this method often requires large equipment with multiple separation columns and is at risk of serious microbial contamination during sampling. Here we propose real-time optical detection of small metabolites using uniform plasmonic nanoparticles monolayers produced by capillary-assisted transfer. We reproducibly fabricate metal nanoparticles monolayers with a diameter of similar to 1 mm for the detection of acetate, butyrate, and glucose by a glass capillary tube. Metal nanoparticles monolayers are not only uniform in terms of average interparticle distance but also structurally stable under dynamic fluidic conditions. The monolayers resistant to fluid shear stress with surface-enhanced Raman scattering are able to reversibly monitor the concentration of acetate and sensitively detect acetate and glucose at levels as low as 10 mu M, which is more than 2 orders of magnitude lower than the concentration range of typical biological gas conversion. In addition, structurally similar metabolites such as acetate and butyrate, when mixed, become distinguishable by our method. English AMER CHEMICAL SOC ENHANCED RAMAN-SPECTROSCOPY FERMENTATION ACETATE SYNGAS SILVER BIOCONVERSION BIOMASS WASTE AU Optical Detection of Small Metabolites for Biological Gas Conversion by using Metal Nanoparticle Monolayers Produced by Capillary-Assisted Transfer Article 10.1021/acs.analchem.9b03439 1 ANALYTICAL CHEMISTRY, v.91, no.20, pp.13152 - 13157 ANALYTICAL CHEMISTRY 91 20 13152 13157 scie scopus 000491219900070 2-s2.0-85072911577 Chemistry, Analytical Chemistry Article ENHANCED RAMAN-SPECTROSCOPY FERMENTATION ACETATE SYNGAS SILVER BIOCONVERSION BIOMASS WASTE AU