Hyung, Sujin Lee, Seung-Ryeol Kim, Yeon Jee Bang, Seokyoung Tahk, Dongha Park, Jong-Chul Suh, Jun-Kyo Francis Jeon, Noo Li 2024-01-19T19:02:51Z 2024-01-19T19:02:51Z 2022-01-25 2019-10 0006-3592 https://pubs.kist.re.kr/handle/201004/119482 Axonal regeneration and remyelination of peripheral motor neurons (MNs) are critical for restoring neuromuscular motor function after injury or peripheral neuropathy. We examined whether optogenetically mediated light stimulation (OMLS) could enhance the axon outgrowth and myelination of MNs using three-dimensional motor neuron-Schwann cell (MN-SC) coculture on a microfluidic biochip. The biochip was designed to allow SCs to interact with the axons of MNs, while preventing direct contact between SCs and the cell bodies of MNs. Following coculture with SCs on the microfluidic biochip, MNs were transfected with a light-sensitive channelrhodopsin gene. Transfected MNs subjected to repeated light stimulation (20 Hz, 1 hr) produced significantly longer axons than nontransfected MNs. OMLS of MNs greatly increased the number of myelin basic protein (MBP)-expressing SCs, promoting the initiation of myelination of MNs. Ultrastructurally, OMLS of MNs markedly enhanced the thickness of the compact myelin sheath around the MN axons such that the average thickness was closer to that of the theoretical estimates in vivo. Thus, the MN-SC coculture model on a microfluidic biochip augmented by OMLS of MNs is a feasible platform for studying the relationship of neuronal activity with regrowth and remyelination. English WILEY Optogenetic neuronal stimulation promotes axon outgrowth and myelination of motor neurons in a three-dimensional motor neuron-Schwann cell coculture model on a microfluidic biochip Article 10.1002/bit.27083 1 BIOTECHNOLOGY AND BIOENGINEERING, v.116, no.10, pp.2425 - 2438 BIOTECHNOLOGY AND BIOENGINEERING 116 10 2425 2438 N scie scopus 000474307700001 2-s2.0-85068518803 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article PERIPHERAL-NERVE ELECTRICAL-STIMULATION NEUROTROPHIC FACTOR CULTURE PLATFORM OPTICAL CONTROL REGENERATION GROWTH motor neuron (MN) myelin optogenetically mediated light stimulation (OMLS) peripheral nerve trauma peripheral neuropathy Schwann cell (SC)