Shin, Jihye Kwon, Yumi Lee, Seonjeong Na, Seungjin Hong, Eun Young Ju, Shinyeong Jung, Hyun-Gyo Kaushal, Prashant Shin, Sungho Back, Ji Hyun Choi, Seon Young Kim, Eun Hee Lee, Su Jin Park, Yae Eun Ahn, Hee-Sung Ahn, Younghee Kabir, Mohammad Humayun Park, Seong-Jun Yang, Won Suk Yeom, Jeonghun Bang, Oh Young Ha, Chul-Won Lee, Jin-Won Kang, Un-Beom Kim, Hye-Jung Park, Kang-Sik Lee, J. Eugene Lee, Ji Eun Kim, Jin Young Kim, Kwang Pyo Kim, Youngsoo Hirano, Hisashi Yi, Eugene C. Cho, Je-Yoel Paek, Eunok Lee, Cheolju 2024-01-19T19:03:03Z 2024-01-19T19:03:03Z 2022-01-10 2019-10 1535-3893 https://pubs.kist.re.kr/handle/201004/119494 We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretomes. cRFP is a small supplementary sequence list of highly abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly. English AMER CHEMICAL SOC NEWLY SYNTHESIZED PROTEINS PROTEOME SERUM Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome Article 10.1021/acs.jproteome.9b00475 1 JOURNAL OF PROTEOME RESEARCH, v.18, no.10, pp.3800 - 3806 JOURNAL OF PROTEOME RESEARCH 18 10 3800 3806 scie scopus 000489200400024 2-s2.0-85072942536 Biochemical Research Methods Biochemistry & Molecular Biology Article NEWLY SYNTHESIZED PROTEINS PROTEOME SERUM secretome common repository of FBS proteins database search