Myung, Da-Bin Han, Hee-Soo Shin, Ji-Sun Park, Ji Yeon Hwang, Han Jun Kim, Hyoung Ja Ahn, Hye Shin Lee, Sun Hee Lee, Kyung-Tae 2024-01-19T19:03:05Z 2024-01-19T19:03:05Z 2021-10-21 2019-10 2072-6643 https://pubs.kist.re.kr/handle/201004/119495 Our previous study showed that hydrangenol isolated from Hydrangea serrata leaves exerts antiphotoaging activity in vitro. In this study, we determined its antiphotoaging effect in UVB-irradiated HR-1 hairless mice. We evaluated wrinkle formation, skin thickness, histological characteristics, and mRNA and protein expression using qRT-PCR and Western blot analysis in dorsal skins. Hydrangenol mitigated wrinkle formation, dorsal thickness, dehydration, and collagen degradation. Hydrangenol increased the expression of involucrin, filaggrin, and aquaporin-3 (AQP3) as well as hyaluronic acid (HA) production via hyaluronidase (HYAL)-1/-2 downregulation. Consistent with the recovery of collagen composition, the expression of Pro-COL1A1 was increased by hydrangenol. Matrix metalloproteinase (MMP)-1/-3, cyclooxygenase-2 (COX-2), and interleukin-6 (IL-6) expression was reduced by hydrangenol. Hydrangenol attenuated the phosphorylation of mitogen-activated protein kinases (MAPKs) including ERK and p38, activator protein 1 (AP-1) subunit, and signal transduction and activation of transcription 1 (STAT1). Hydrangenol upregulated the expression of nuclear factor-E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO-1), glutamate cysteine ligase modifier subunit (GCLM), and glutamate cysteine ligase catalysis subunit (GCLC). Taken together, our data suggest that hydrangenol can prevent wrinkle formation by reducing MMP and inflammatory cytokine levels and increasing the expression of moisturizing factors and antioxidant genes. English MDPI Hydrangenol Isolated from the Leaves of Hydrangea serrata Attenuates Wrinkle Formation and Repairs Skin Moisture in UVB-Irradiated Hairless Mice Article 10.3390/nu11102354 1 NUTRIENTS, v.11, no.10 NUTRIENTS 11 10 scie scopus 000498227300095 2-s2.0-85072914277 Nutrition & Dietetics Nutrition & Dietetics Article INDUCED DOWN-REGULATION HUMAN KERATINOCYTES EXPRESSION RADIATION HYALURONIDASE AQUAPORIN-3 CELLS ACID NRF2 METALLOPROTEINASES hydrangenol ultraviolet B photoaging MMPs collagen skin moisture wrinkle formation MAPK AP-1