Lim, Seungho Yoon, Hong Yeol Jang, Hee Jeon Song, Sukyung Kim, Woojun Park, Jooho Lee, Kyung Eun Jeon, Sangmin Lee, Sangmin Lim, Dong-Kwon Kim, Byung-Soo Kim, Dong-Eog Kim, Kwangmeyung 2024-01-19T19:03:08Z 2024-01-19T19:03:08Z 2021-09-05 2019-10 1936-0851 https://pubs.kist.re.kr/handle/201004/119498 Noninvasive and precise stem cell tracking after transplantation in living subject is very important to monitor both stem cell destinations and their in vivo fate, which is closely related to their therapeutic efficacy. Herein, we developed bicyclo[6.1.0]nonyne (BCN)-conjugated glycol chitosan nanoparticles (BCN-NPs) as a delivery system of dual-modal stem cell imaging probes. Near-infrared fluorescent (NIRF) dye CyS.S was chemically conjugated to the BCN-NPs, and then oleic acidcoated superparamagnetic iron oxide nanoparticles (OA-Fe3O4 NPs) were encapsulated into BCN-NPs, resulting in Cy5.5-labeled and OA-Fe3O4 NP-encapsulated BCN-NPs (BCN-dual-NPs). For bioorthogonal labeling of human adipose-derived mesenchymal stem cells (hMSCs), first, hMSCs were treated with tetra-acetylated N-azidoacetyl-D-mannosamine (Ac(4)ManNAz) for generating azide (-N-3) groups onto their surface via metabolic glycoengineering. Second, azide groups on the cell surface were successfully chemically labeled with BCN-dual-NPs via bioorthogonal click chemistry in vitro. This bioorthogonal labeling of hMSCs could greatly increase the cell labeling efficiency, safety, and imaging sensitivity, compared to only nanoparticle-derived labeling technology. The dual-modal imaging-guided precise tracking of bioorthogonally labeled hMSCs was tested in the photothrombotic stroke mouse model via intraparenchymal injection. Finally, BCN-dual-NPs-labeled hMSCs could be effectively tracked by their migration from the implanted site to the brain stroke lesion using NIRF/T-2 -weighted magnetic resonance (MR) dual-modal imaging for 14 days. Our observation would provide a potential application of bioorthogonally labeled stem cell imaging in regenerative medicine by providing safety and high labeling efficiency in vitro and in vivo. English AMER CHEMICAL SOC GLYCOL CHITOSAN NANOPARTICLES COPPER-FREE RAT MODEL TRANSPLANTATION DELIVERY BIODISTRIBUTION PEPTIDES Dual-Modal Imaging-Guided Precise Tracking of Bioorthogonally Labeled Mesenchymal Stem Cells in Mouse Brain Stroke Article 10.1021/acsnano.9b02173 1 ACS NANO, v.13, no.10, pp.10991 - 11007 ACS NANO 13 10 10991 11007 scie scopus 000492801600013 2-s2.0-85073219892 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article GLYCOL CHITOSAN NANOPARTICLES COPPER-FREE RAT MODEL TRANSPLANTATION DELIVERY BIODISTRIBUTION PEPTIDES metabolic engineering bioorthogonal click chemistry imaging probe stem cell tracking brain stroke dual-modal imaging