Woo, Junsung Yang, Hyejin Yoon, Minseok Gadhe, Changdev G. Pae, Ae Nim Cho, Suengmok Lee, C. Justin 2024-01-19T19:03:42Z 2024-01-19T19:03:42Z 2021-09-04 2019-10 1226-2560 https://pubs.kist.re.kr/handle/201004/119532 3-Carene, a bicyclic monoterpene, is one of the major components of the pine tree essential oils. It has been reported that, in addition to its known properties as a phytoncide, 3-carene has anti-inflammatory, antimicrobial, and anxiolytic effects. We have previously demonstrated that a-pinene, the major component of pine tree, has a hypnotic effect through GABA(A)-benzodiazepine (BZD) receptors. However, a hypnotic effect of 3-Caren has not been studied yet. Here, we report that oral administration of 3-carene increases the sleep duration and reduces sleep latency in pentobarbital- induced sleep test. 3-Carene potentiates the GABA(A) receptor-mediated synaptic responses by prolonging the decay time constant of inhibitory synaptic responses. These enhancing effects of 3-carene are reproduced by zolpidem, a modulator for GABA(A)-BZD receptor, and fully inhibited by flumazenil, an antagonist for GABA(A)-BZD receptor. The molecular docking of 3-carene to the BZD site of GABA(A) protein structure, suggests that 3-carene binds to the BM site of alpha 1 and Upsilon 2 subunits of GABA(A)- BZD receptor. These results indicate that, similar to alpha-pinene, 3-carene shows a sleep-enhancing effect by acting as a positive modulator for GABA(A)-BZD receptor. English KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE EYE-MOVEMENT SLEEP SLOW-WAVE SLEEP ESSENTIAL OILS CHEMICAL-COMPOSITION GABA(A) RECEPTORS BUPLEURUM-GIBRALTARICUM BENZODIAZEPINE BINDING ANTIOXIDANT ACTIVITY SUBUNIT ZOLPIDEM 3-Carene, a Phytoncide from Pine Tree Has a Sleep-enhancing Effect by Targeting the GABA(A)-benzodiazepine Receptors Article 10.5607/en.2019.28.5.593 1 EXPERIMENTAL NEUROBIOLOGY, v.28, no.5, pp.593 - 601 EXPERIMENTAL NEUROBIOLOGY 28 5 593 601 scie scopus kci ART002521862 000496478400005 2-s2.0-85077980114 Medicine, Research & Experimental Neurosciences Research & Experimental Medicine Neurosciences & Neurology Article EYE-MOVEMENT SLEEP SLOW-WAVE SLEEP ESSENTIAL OILS CHEMICAL-COMPOSITION GABA(A) RECEPTORS BUPLEURUM-GIBRALTARICUM BENZODIAZEPINE BINDING ANTIOXIDANT ACTIVITY SUBUNIT ZOLPIDEM 3-carene Sleep GABA(A)-BZD receptor Phytoncide