Park, Jun-Hee Song, Zhiquan Lee, Ga-Yeon Jeong, Seong-Min Kang, Min-Jung Pyun, Jae-Chul 2024-01-19T19:04:06Z 2024-01-19T19:04:06Z 2021-09-05 2019-09-27 0003-2670 https://pubs.kist.re.kr/handle/201004/119554 Highly N-doped SiC was presented as an optimal electrode for electrochemical immunoassays with a far higher sensitivity than chemiluminescence detection. As the first step, the electrochemical properties of highly N-doped SiC, such as the double-layer capacitance (C-dl), rate constant for electron transfer (k(app)) and ideal polarizable potential range (electrochemical window) were analyzed and compared with those of Au, Pt, and graphite electrodes. The highly N-doped SiC electrode was used for the quantification of oxidized 3,3',5,5'-tetramethylbenzidine (TMB) which was widely used as chromogenic substrate for commercialized immunoassay kits. In order to enhance the sensitivity for the quantification of the oxidized TMB the chronoamperometry was applied to avoid the background current of i-V measurement. Finally, the chronoamperometry based on the highly N-doped SiC electrode was applied to commercial immunoassay kits for the medical diagnosis of the human immunodeficiency virus (HIV) and the human hepatitis B surface antigen (hHBsAg). The chronoamperometric measurement based on the highly N-doped SiC electrode was proved to detect at far lower limits in comparison with the conventional optical density measurement as well as the chemiluminescence assay based on luminol as a chemiluminescent probe. (C) 2019 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV TRANSFER KINETICS HIGH-TEMPERATURE SEMICONDUCTOR GOLD OXIDATION BEHAVIOR OXYGEN Hypersensitive electrochemical immunoassays based on highly N-doped silicon carbide (SiC) electrode Article 10.1016/j.aca.2019.04.054 1 ANALYTICA CHIMICA ACTA, v.1073, pp.30 - 38 ANALYTICA CHIMICA ACTA 1073 30 38 scie scopus 000469211400003 2-s2.0-85065037443 Chemistry, Analytical Chemistry Article TRANSFER KINETICS HIGH-TEMPERATURE SEMICONDUCTOR GOLD OXIDATION BEHAVIOR OXYGEN SiC Immunoassay Chronoamperometry Chemiluminescence Medical diagnosis