Kim, Jong-Jin Lee, Yong-An Su, Dongdong Lee, Jungyeol Park, Sung-Jin Kim, Beomsue Lee, Jia Hui Jane Liu, Xiao Kim, Seong Soon Bae, Myung Ae Lee, Jun-Seok Hong, Seong Cheol Wang, Lu Samanta, Animesh Kwon, Haw-Young Choi, So-Young Kim, Jun-Young Yu, Young Hyun Ha, Hyung-Ho Wang, Zhenxun Tam, Wai Leong Lim, Bing Kang, Nam-Young Chang, Young-Tae 2024-01-19T19:04:16Z 2024-01-19T19:04:16Z 2021-09-04 2019-09-18 0002-7863 https://pubs.kist.re.kr/handle/201004/119563 Tumor initiating cells (TIC) are resistant to conventional anticancer therapy and associated with metastasis and relapse in cancer. Although various TIC markers and their antibodies have been proposed, it is limited to the use of antibodies for in vivo imaging or treatment of TIC. In this study, we discovered heme oxygenase 2 (HMOX2) as a novel biomarker for TIC and developed a selective small molecule probe TiNIR (tumor initiating cell probe with near infrared). TiNIR detects and enriches the functionally active TIC in human lung tumors, and through the photoacoustic property, TiNIR also visualizes lung TIC in the patient-derived xenograft (PDX) model. Furthermore, we demonstrate that TiNIR inhibits tumor growth by blocking the function of HMOX2, resulting in significantly increased survival rates of the cancer model mice. The novel therapeutic target HMOX2 and its fluorescent ligand TiNIR will open a new path for the molecular level of lung TIC diagnosis and treatment. English AMER CHEMICAL SOC CANCER STEM-CELLS FLUORESCENT CHEMICAL PROBE REAL-TIME HEME CHALLENGES LIBRARY DRUG DYE A Near-Infrared Probe Tracks and Treats Lung Tumor Initiating Cells by Targeting HMOX2 Article 10.1021/jacs.9b06068 1 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.141, no.37, pp.14673 - 14686 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141 37 14673 14686 scie scopus 000487180200031 2-s2.0-85072362131 Chemistry, Multidisciplinary Chemistry Article CANCER STEM-CELLS FLUORESCENT CHEMICAL PROBE REAL-TIME HEME CHALLENGES LIBRARY DRUG DYE fluorescent probe Cancer Stem cell