Noh, Yong Kwan Da Costa, Avelino Dos Santos Park, Yong Seek Du, Ping Kim, Ik-Hwan Park, Kwideok 2024-01-19T19:04:51Z 2024-01-19T19:04:51Z 2021-09-05 2019-09-01 0144-8617 https://pubs.kist.re.kr/handle/201004/119597 Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of a-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold. English ELSEVIER SCI LTD IN-VITRO MECHANICAL-PROPERTIES POTENTIAL SCAFFOLD STROMAL CELLS BIOCOMPATIBILITY NANOCOMPOSITE ANGIOGENESIS BIOMATERIALS MATRIX Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells Article 10.1016/j.carbpol.2019.05.039 1 CARBOHYDRATE POLYMERS, v.219, pp.210 - 218 CARBOHYDRATE POLYMERS 219 210 218 scie scopus 000469334500023 2-s2.0-85065639152 Chemistry, Applied Chemistry, Organic Polymer Science Chemistry Polymer Science Article IN-VITRO MECHANICAL-PROPERTIES POTENTIAL SCAFFOLD STROMAL CELLS BIOCOMPATIBILITY NANOCOMPOSITE ANGIOGENESIS BIOMATERIALS MATRIX Bacterial cellulose (BC) Collagen Umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) Osteogenesis Bone regeneration