Han, Won Bae Kang, Dong-Hyun Na, Jung-Hyun Yu, Yeon Gyu Kim, Tae Song 2024-01-19T19:30:22Z 2024-01-19T19:30:22Z 2021-09-05 2019-09 0956-5663 https://pubs.kist.re.kr/handle/201004/119611 The bio-sensory organs of living creatures have evolved to have the best sensing performance. They have 3-dimensional protrusions that have large surface areas to accommodate a large number of membrane proteins such as ion channels and G-protein coupled receptors, resulting in high sensitivity and specificity to target molecules. From the perspective of mimicking this system, BLM, which has been used extensively as a platform for a single nanopore-based sensing systems, has some limitations, i.e., some residual solvent, low mechanical stability, small surface area for appropriate stability, and difficulty in high-throughput fabrication. Herein, to eliminate these limitations, a solvent-free, size-controllable, 3-dimensional free-standing lipid bilayer (3DFLB) structure array with high stability (similar to 130 h) and high density (similar to 300,000 cm(-2)) is proposed, and its structural advantages for efficient and rapid protein reconstitution, compared to BLM, is demonstrated by human 5-HT3A receptor assay as well as alpha-hemolysin assay. A continuous process of 3DFLB array fabrication, 5-HT3A reconstitution, and 5-HT detections in a microfluidic channel proves the applicability of the proposed structures as a highly-sensitive sensing platform mimicking bio-sensory organs. English Pergamon Press Ltd. Enhancement of membrane protein reconstitution on 3D free-standing lipid bilayer array in a microfluidic channel Article 10.1016/j.bios.2019.111404 1 Biosensors and Bioelectronics, v.141 Biosensors and Bioelectronics 141 N scie scopus 000486132800036 2-s2.0-85067079998 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article UNILAMELLAR VESICLES TRANSPORT FUSION RECORDINGS STABILITY RECEPTOR BIOSENSOR APERTURES Lipid 3-Dimensional lipid structure Free-standing lipid bilayer Protein reconstitution Membrane protein