Kim, Eun-Ju Choi, Mingi Park, Hyeon Yeong Hwang, Ji Young Kim, Hyung-Eun Hong, Seok Won Lee, Jaesang Yong, Kijung Kim, Wooyul 2024-01-19T19:31:08Z 2024-01-19T19:31:08Z 2021-09-04 2019-09 2045-2322 https://pubs.kist.re.kr/handle/201004/119655 To overcome the conventional limitation of TiO2 disinfection being ineffective under light-free conditions, TiO2 nanowire films (TNWs) were prepared and applied to bacterial disinfection under dark and UV illumination. TNW exhibited much higher antibacterial efficiencies against Escherichia coli (E. coli) under dark and UV illumination conditions compared to TiO2 nanoparticle film (TNP) which was almost inactive in the dark, highlighting the additional contribution of the physical interaction between bacterial membrane and NWs. Such a physical contact-based antibacterial activity was related to the NW geometry such as diameter, length, and density. The combined role of physical puncture and photocatalytic action in the mechanism underlying higher bactericidal effect of TNW was systematically examined by TEM, SEM, FTIR, XPS, and potassium ion release analyses. Moreover, TNW revealed antimicrobial activities in a broad spectrum of microorganisms including Staphylococcus aureus and MS2 bacteriophage, antibiofilm properties, and good material stability. Overall, we expect that the free-standing and antimicrobial TNW is a promising agent for water disinfection and biomedical applications in the dark and/or UV illumination. English NATURE PUBLISHING GROUP Thorn-like TiO2 nanoarrays with broad spectrum antimicrobial activity through physical puncture and photocatalytic action Article 10.1038/s41598-019-50116-0 1 SCIENTIFIC REPORTS, v.9 SCIENTIFIC REPORTS 9 N scie scopus 000487216300017 2-s2.0-85072582906 Multidisciplinary Sciences Science & Technology - Other Topics Article ESCHERICHIA-COLI FTIR SPECTROSCOPY ATR-FTIR ANATASE LIGHT DISINFECTION BROOKITE ANTIBACTERIAL NANOPARTICLES DEGRADATION TiO2 nanowire array Morphological engineering Antimicrobial properties Dark conditions Physical puncture