Lee, Sanghee Cho, Wansang Hong, Sungyoul Yi, Sihyeong Kim, Heejun Baek, Soo Yeon Park, Hankum Jung, Jinjoo Shin, Young Kee Park, Seung Bum 2024-01-19T19:31:26Z 2024-01-19T19:31:26Z 2021-09-02 2019-08-21 1477-0520 https://pubs.kist.re.kr/handle/201004/119672 Selective bioactive compounds have emerged as major players in chemical biology for their potential in disrupting diverse biological pathways with minimal adverse effects. Using phenotypic screening, we identified an anti-cancer agent, SB2001, with a highly specific cytotoxicity toward HeLa human cervical cancer cells. The subsequent mechanistic study revealed that SB2001 induced apoptotic cell death through restoring p53 function and suppressed the human papillomavirus (HPV)-mediated oncoprotein signaling pathway via oxidative damage in HeLa cells. SB2001 also selectively induced HeLa-specific tumor regression without any adverse effects in an in vivo tumor xenograft model, demonstrating its potential as a promising chemical probe. English ROYAL SOC CHEMISTRY TARGET IDENTIFICATION P53 INHIBITOR MECHANISM APOPTOSIS BIOLOGY ARREST CANCER Phenotype-based discovery of a HeLa-specific cytotoxic molecule that downregulates HPV-mediated signaling pathways via oxidative damage Article 10.1039/c9ob01341e 1 ORGANIC & BIOMOLECULAR CHEMISTRY, v.17, no.31, pp.7388 - 7397 ORGANIC & BIOMOLECULAR CHEMISTRY 17 31 7388 7397 scie scopus 000479062100016 2-s2.0-85070481723 Chemistry, Organic Chemistry Article TARGET IDENTIFICATION P53 INHIBITOR MECHANISM APOPTOSIS BIOLOGY ARREST CANCER