Jung, Hyunkyung Park, Sungjo Gunassekaran, Gowri Rangaswamy Jeon, Mansik Cho, Young-Eun Baek, Moon-Chang Park, Jae Yong Shim, Gayong Oh, Yu-Kyoung Kim, In-San Kim, Chulhong Lee, Byungheon 2024-01-19T19:31:36Z 2024-01-19T19:31:36Z 2021-09-02 2019-08-15 0008-5472 https://pubs.kist.re.kr/handle/201004/119681 The lack of molecular targets and targeting probes remains a major drawback for targeted imaging and drug delivery in lung cancer. In this study, we exploited in vivo phage display to identify a novel targeting probe that homes to the tumor in a K-ras(LA2) mutant mouse lung cancer model. Compared with other candidate peptides selected from 5 rounds of phage display, the CRQTKN peptide homed to tumor nodules in the lung of mutant mice at higher levels. Photoacoustic tomography of mutant mice detected lung tumors via tumor homing of the near-infrared fluorescence dye-labeled CRQTKN peptide. Ex vivo photoacoustic images of isolated organs further demonstrated tumor homing of the CRQTKN peptide, whereas minimal accumulation was observed in control organs, such as the liver. Compared with untargeted liposomes and doxorubicin, doxorubicin-loaded liposomes whose surface was modified with the CRQTKN peptide more efficiently delivered doxorubicin and reduced the number or size of tumor lesions in K-ras(LA2) mutant mice. Analysis of hematologic parameters and liver and kidney function showed no significant systemic side effects by the treatments. Affinity-based identification was used to detect TNF receptor superfamily member 19L (TNFRSF19L), which was upregulated in lung tumors of mutant mice, as the receptor for the CRQTKN peptide. In conclusion, these results suggest that the CRQTKN peptide is a promising targeting probe for photoacoustic-guided detection and drug delivery to lung cancer, and acts by binding to TNFRSF19L. Significance: These findings present a new tumor-targeting probe for photoacoustic-guided detection and drug delivery. English AMER ASSOC CANCER RESEARCH PHAGE DISPLAY FAMILY-MEMBERS TNF RECEPTOR MOUSE MODEL CANCER TOMOGRAPHY GROWTH NANOPARTICLES LOCALIZATION PENETRATION A Peptide Probe Enables Photoacoustic-Guided Imaging and Drug Delivery to Lung Tumors in K-ras(LA2) Mutant Mice Article 10.1158/0008-5472.CAN-18-3089 1 CANCER RESEARCH, v.79, no.16, pp.4271 - 4282 CANCER RESEARCH 79 16 4271 4282 scie scopus 000481961100024 2-s2.0-85070724291 Oncology Oncology Article PHAGE DISPLAY FAMILY-MEMBERS TNF RECEPTOR MOUSE MODEL CANCER TOMOGRAPHY GROWTH NANOPARTICLES LOCALIZATION PENETRATION