Ryu, Seuk-Min Hur, Junseok W. Kim, Kyoungmi 2024-01-19T19:31:54Z 2024-01-19T19:31:54Z 2022-01-25 2019-08 1976-6696 https://pubs.kist.re.kr/handle/201004/119698 The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction. English 생화학분자생물학회 Evolution of CRISPR towards accurate and efficient mammal genome engineering Article 10.5483/BMBRep.2019.52.8.149 1 BMB Reports, v.52, no.8, pp.475 - 481 BMB Reports 52 8 475 481 Y scie scopus kci ART002494785 000484007700001 2-s2.0-85071712171 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Review STRAND BREAK REPAIR HOMOLOGY-DIRECTED REPAIR ONE-STEP GENERATION DNA-REPAIR KNOCK-IN BASE PATHWAY RECOMBINATION INHIBITION PRECISION CRISPR DNA double-strand break Genome editing HDR NHEJ