Storace, Douglas A. Cohen, Lawrence B. Choi, Yunsook 2024-01-19T19:33:03Z 2024-01-19T19:33:03Z 2022-01-10 2019-07-31 1662-5102 https://pubs.kist.re.kr/handle/201004/119762 Genetically encoded voltage indicators (GEVIs) are fluorescent protein reporters of membrane potential. These tools can, in principle, be used to monitor the neural activity of genetically distinct cell types in the brain. Although introduced in 1997, they have been a challenge to use to study intact neural circuits due to a combination of small signal-tonoise ratio, slow kinetics, and poor membrane expression. New strategies have yielded novel GEVIs such as ArcLight, which have improved properties. Here, we compare the in vivo properties of ArcLight with Genetically Encoded Calcium Indicators (GECIs) in the mouse olfactory bulb. We show how voltage imaging can be combined with organic calcium sensitive dyes to measure the input-output transformation of the olfactory bulb. Finally, we demonstrate that ArcLight can be targeted to olfactory bulb interneurons. The olfactory bulb contributes substantially to the perception of the concentration invariance of odor recognition. English FRONTIERS MEDIA SA CENTER-SURROUND INHIBITION NEURAL ACTIVITY OPTICAL ELECTROPHYSIOLOGY ODOR REPRESENTATIONS FLUORESCENT PROTEIN ACTION-POTENTIALS NEURONS EXPRESSION NETWORK CELLS Using Genetically Encoded Voltage Indicators (GEVIs) to Study the Input-Output Transformation of the Mammalian Olfactory Bulb Article 10.3389/fncel.2019.00342 1 FRONTIERS IN CELLULAR NEUROSCIENCE, v.13 FRONTIERS IN CELLULAR NEUROSCIENCE 13 scie scopus 000478034600001 2-s2.0-85072721512 Neurosciences Neurosciences & Neurology Article CENTER-SURROUND INHIBITION NEURAL ACTIVITY OPTICAL ELECTROPHYSIOLOGY ODOR REPRESENTATIONS FLUORESCENT PROTEIN ACTION-POTENTIALS NEURONS EXPRESSION NETWORK CELLS ArcLight odors olfactory bulb mouse perception GEVIs GECIs