Erdene-Ochir, Erdenedolgor Shin, Bok-Kyu Kwon, Byeori Jung, Choonkyun Pan, Cheol-Ho 2024-01-19T19:33:19Z 2024-01-19T19:33:19Z 2021-09-02 2019-07-09 2045-2322 https://pubs.kist.re.kr/handle/201004/119776 Although diatoms have been extensively studied as bioreactors, only a limited number of efficient gene promoters are available. Therefore, the development of new endogenous promoters is important for the heterologous production of a variety of recombinant proteins. Herein, we identified the most abundant secreted protein in Phaeodactylum tricornutum, designated 'highly abundant secreted protein 1' (HASP1), and characterised the activities of its promoter and signal peptide using green fluorescent protein (GFP) as a reporter. The HASP1 promoter strongly drove GFP expression during all growth phases of P. tricornutum in culture, in contrast to the commonly used fcpA promoter, which is less active during the stationary phase. The HASP1 signal peptide was also sufficient for facilitating efficient secretion of GFP by P. tricornutum. Our findings suggest that both the promoter and the signal peptide of HASP1 can be utilized as novel tools for the overexpression and secretion of recombinant proteins in P. tricornutum. English NATURE PUBLISHING GROUP BETA-CARBONIC ANHYDRASE GENE-EXPRESSION IN-SILICO TRANSFORMATION PROTEINS SYSTEM BACILLARIOPHYCEAE BIOSYNTHESIS COMPARTMENTS SEQUENCES Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum Article 10.1038/s41598-019-45786-9 1 SCIENTIFIC REPORTS, v.9 SCIENTIFIC REPORTS 9 scie scopus 000474505900052 2-s2.0-85068736217 Multidisciplinary Sciences Science & Technology - Other Topics Article BETA-CARBONIC ANHYDRASE GENE-EXPRESSION IN-SILICO TRANSFORMATION PROTEINS SYSTEM BACILLARIOPHYCEAE BIOSYNTHESIS COMPARTMENTS SEQUENCES HASP1 promoter signal peptide Phaeodactylum tricornutum