Oh, Hyun Ju Kim, Ki-Yeon Lee, Kyung Min Lee, Sun-Mi Gong, Gyeongtaek Oh, Min-Kyu Um, Youngsoon 2024-01-19T19:34:44Z 2024-01-19T19:34:44Z 2021-09-02 2019-07 1226-086X https://pubs.kist.re.kr/handle/201004/119858 Butyric acid-producing strain Clostridium tyrobutyricum ATCC25755 produces acetic acid as a byproduct, which lowers butyric acid yield and selectivity. Supplementing glycerol would be a rational approach to enhance a NADH-requiring metabolite butyric acid selectivity by increasing NADH availability; however, there has been no report butyric acid production using glycerol as either a sole carbon source or a co-substrate with C. tyrobutyricum. In this study, glycerol was supplemented along with sugar to increase NADH availability facilitating the production of butyric acid. C. tyrobutyricum was found to simultaneously consume glucose and glycerol only when acetic acid was present. Notably, acetic acid was not produced but consumed. The amount of glycerol consumption was linearly dependent on the amount of acetic acid consumption. When mixed sugars (glucose 80 g/L and xylose 30 g/L) and crude glycerol were used, not only exogenous acetic acid consumption but also acetic acid reassimilation were clearly observed, resulting in significantly enhanced butyric acid production (54.79 g/L), yield (0.464 g/g), and selectivity (98.3%) compared to sugar-only fermentation (production, 35.42 g/L; yield, 0.339 g/g; selectivity, 69.1%). Therefore, this study would pave the way for selective and cost-effective butyric acid production using sugars and glycerol from biodiesel facility. (C) 2019 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. English 한국공업화학회 Butyric acid production with high selectivity coupled with acetic acid consumption in sugar-glycerol mixture fermentation by Clostridium tyrobutyricum ATCC25755 Article 10.1016/j.jiec.2019.01.047 1 Journal of Industrial and Engineering Chemistry, v.75, pp.44 - 51 Journal of Industrial and Engineering Chemistry 75 44 51 N scie scopus kci ART002492929 000466248600004 2-s2.0-85061797959 Chemistry, Multidisciplinary Engineering, Chemical Chemistry Engineering Article CRUDE GLYCEROL BUTANOL PRODUCTION DELETED MUTANT ACETOBUTYLICUM HYDROLYSATE BIODIESEL GLUCOSE CONSTRUCTION CONVERSION SUBSTRATE Butyric acid Glycerol Glucose Acetic acid reassimilation Clostridium tyrobutyricumATCC 25755