Oh, Hyun-A Lee, Hyunbeom Kang, Keon Wook Im, Ji Hye Kim, Donghak Yang, Hyun Ok Jung, Byung Hwa 2024-01-19T20:01:29Z 2024-01-19T20:01:29Z 2021-09-02 2019-06 0269-3879 https://pubs.kist.re.kr/handle/201004/119931 Traditional herbal medicine consists of multiple components. There are interactions among the components, which affect both potency and toxicity. The preparation of herbal medicines can be a cause of interactions between multicomponents in herbs. To demonstrate the differences in multiherb interactions based on the preparation methods, the changes in the active components in the different preparations of Socheongryong-tang (SCRT) were evaluated using metabolomics profiling. We performed multicomponent profiling of the decoction of SCRT (SCRTD) and individual herb mixture (SCRTM) using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Active compounds from SCRTD and SCRTM were identified using multivariate analysis, and the activities between the two groups were compared. We also evaluated the anti-inflammatory effect of SCRT through investigating the protein expression of iNOS and COX-2 in lipopolysaccharide-induced macrophage RAW 264.7 cells in both groups. From the multivariate analysis, 53 active compounds that have different intensities between SCRTD and SCRTM were identified. The intensities of those components, such as ephedrines, glycyrrhizic acid, 6-gingerol and (2E,4E,8Z,10E)-N-isobutyl-2,4,8,10-dodecatetraenamide, which is newly identified in Asiasarum heterotropoides, were mostly higher in SCRTD than in SCRTM, which was related to the anti-inflammatory effect. From the iNOS inhibition test, it was found that SCRTD had a stronger anti-inflammatory effect than SCRTM. It was demonstrated that multicomponent interactions can be changed by the preparation method, and finally the anti-inflammatory effect in SCRT can be affected. English WILEY TRADITIONAL CHINESE MEDICINE QING-LONG-TANG ANTIINFLAMMATORY ACTIVITY PAEONIA-LACTIFLORA MASS-SPECTROMETRY CINNAMOMUM-CASSIA CONSTITUENTS INFLAMMATION SCHISANDRIN INHIBITION Identification of interactions between multiple components in Socheongryong-tang using a plant profiling approach Article 10.1002/bmc.4500 1 BIOMEDICAL CHROMATOGRAPHY, v.33, no.6 BIOMEDICAL CHROMATOGRAPHY 33 6 scie scopus 000467860100015 2-s2.0-85061931996 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Pharmacology & Pharmacy Biochemistry & Molecular Biology Chemistry Pharmacology & Pharmacy Article TRADITIONAL CHINESE MEDICINE QING-LONG-TANG ANTIINFLAMMATORY ACTIVITY PAEONIA-LACTIFLORA MASS-SPECTROMETRY CINNAMOMUM-CASSIA CONSTITUENTS INFLAMMATION SCHISANDRIN INHIBITION multicomponent interactions plant profiling approach Socheongryong-tang UPLC-QTOF-MS