Bi, Baibin Choi, Han-Pil Hyeon, Seung Jae Sun, Shengnan Su, Ning Liu, Yuguang Lee, Junghee Kowall, Neil W. McKee, Ann C. Yang, Jing-Hua Ryu, Hoon 2024-01-19T20:01:30Z 2024-01-19T20:01:30Z 2021-09-02 2019-06 1226-2560 https://pubs.kist.re.kr/handle/201004/119932 Chronic traumatic encephalopathy (CTE) is a distinct neurodegenerative disease that associated with repetitive head trauma. CTE is neuropathologically defined by the perivascular accumulation of abnormally phosphorylated tau protein in the depths of the sulci in the cerebral cortices. In advanced CTE, hyperphosphorylated tau protein deposits are found in widespread regions of brain, however the mechanisms of the progressive neurodegeneration in CTE are not fully understood. In order to identify which proteomic signatures are associated with CTE, we prepared RIPA-soluble fractions and performed quantitative proteomic analysis of postmortem brain tissue from individuals neuropathologically diagnosed with CTE. We found that axonal guidance signaling pathway-related proteins were most significantly decreased in CTE. Immunohistochemistry and Western blot analysis showed that axonal signaling pathway-related proteins were down regulated in neurons and oligodendrocytes and neuron-specific cytoskeletal proteins such as TUBB3 and CFLI were reduced in the neuropils and cell body in CTE. Moreover, oligodendrocyte-specific proteins such as MAG and TUBB4 were decreased in the neuropils in both gray matter and white matter in CTE, which correlated with the degree of axonal injury and degeneration. Our findings indicate that deregulation of axonal guidance proteins in neurons and oligodendrocytes is associated with the neuropathology in CTE. Together, altered axonal guidance proteins may be potential pathological markers for CTE. English KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE AMYLOID-BETA ACCUMULATION HEAD-INJURY ALZHEIMERS-DISEASE RISK-FACTOR STRETCH-INJURY TAU PROTEINS AXOTOMY IMPACT EPIDEMIOLOGY Quantitative Proteomic Analysis Reveals Impaired Axonal Guidance Signaling in Human Postmortem Brain Tissues of Chronic Traumatic Encephalopathy Article 10.5607/en.2019.28.3.362 1 EXPERIMENTAL NEUROBIOLOGY, v.28, no.3, pp.362 - 375 EXPERIMENTAL NEUROBIOLOGY 28 3 362 375 scie scopus kci ART002485846 000474466200006 2-s2.0-85069449288 Medicine, Research & Experimental Neurosciences Research & Experimental Medicine Neurosciences & Neurology Article AMYLOID-BETA ACCUMULATION HEAD-INJURY ALZHEIMERS-DISEASE RISK-FACTOR STRETCH-INJURY TAU PROTEINS AXOTOMY IMPACT EPIDEMIOLOGY Chronic traumatic encephalopathy (CTE) Quantitative proteomics Axonal guidance Oligodendrocyte Neuron Neurodegeneration