Kim, Jung Min Kim, Won Jin Kim, Mi Yeon Kim, Kwang Pyo Sim, Sang Jun Kim, Sang Kyung 2024-01-19T20:01:44Z 2024-01-19T20:01:44Z 2021-09-02 2019-06 1976-0280 https://pubs.kist.re.kr/handle/201004/119941 Reverse transcription - quantitative polymerase chain reaction (RT-qPCR) is conventionally used method to analyze oncogenes, infected DNAs, and mutated tumor associated genes. However it is hard to detect rare genetic targets since the disturbance of undesired amplification often overrides the reaction of very few targets in a myriad of interfering genes. To solve the limitation, we developed primerimmobilized network (PIN) probe RT-qPCR which can detect target RNA with high selectivity and sensitivity. To conduct PIN probe RT-qPCR with high efficiency, design of probe, concentration of immobilized probe and qPCR condition were optimized. The LOD of PIN probe RT-qPCR was 40pg per particle with 89.2% efficiency. When extremely low concentration of RNA was used, result of PIN probe RTqPCR was showed on/off signal. Also, target was confirmed only in the on particle. The interference effects by non-target PCR products were minimized by target-capturing and washing process in the RT. Using PIN probe RT-qPCR, we successfully detected target RNA in a sample which has a ratio of 1:100,000 (positive RNA : negative RNA). English KOREAN BIOCHIP SOCIETY-KBCS FUSION TRANSCRIPTS RESIDUAL DISEASE LEUKEMIA PCR ASSAY Development of Hydrogel Microparticle based RT-qPCR for Advanced Detection of BCR-ABL1 Transcripts Article 10.1007/s13206-018-3209-9 1 BIOCHIP JOURNAL, v.13, no.2, pp.182 - 190 BIOCHIP JOURNAL 13 2 182 190 scie scopus kci ART002473465 000472838700009 2-s2.0-85067884913 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article FUSION TRANSCRIPTS RESIDUAL DISEASE LEUKEMIA PCR ASSAY N Real-time PCR RT-qPCR Hydrogel microparticle TaqMan probe Chronic myeloid leukemia BCR-ABL