Hong, Gyu-Sang Lee, Sung Hoon Lee, Byeongjun Choi, Jae Hyouk Oh, Soo-Jin Jang, Yongwoo Hwang, Eun Mi Kim, Hyungsup Jung, Jooyoung Kim, In-Beom Oh, Uhtaek 2024-01-19T20:01:47Z 2024-01-19T20:01:47Z 2021-09-02 2019-06 0027-8424 https://pubs.kist.re.kr/handle/201004/119943 Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca2+-activated chloride channel, mediates the Ca2+-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGC5 of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for BDNF-induced process extension in RGCs. More importantly, Ano/-deficient mice exhibited disrupted cortical layers and reduced cortical thickness. We thus conclude that the regulation of RGC process extension by ANO1 contributes to the normal formation of mouse embryonic brain. English NATL ACAD SCIENCES ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain Article 10.1073/pnas.1901067116 1 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.116, no.25, pp.12494 - 12499 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116 25 12494 12499 N scie scopus 000471887900059 2-s2.0-85067619431 Multidisciplinary Sciences Science & Technology - Other Topics Article ACTIVATED CHLORIDE CHANNEL NEURAL STEM-CELLS TRANSMEMBRANE PROTEIN TMEM16A EXPRESSION GROWTH GABA DIFFERENTIATION PROLIFERATION EVOLUTION Anoctamin 1 TMEM16A neural stem cell radial glial cell cortical development