Kim, Yoon Hwan Shim, Hyun Soo Kim, Kyoung Heon Lee, Junghee Chung, Bong Chul Kowall, Neil W. Ryu, Hoon Lee, Jeongae 2024-01-19T20:01:54Z 2024-01-19T20:01:54Z 2021-09-02 2019-06 1226-2560 https://pubs.kist.re.kr/handle/201004/119950 Despite significant advances in neuroscience research over the past several decades, the exact cause of AD has not yet fully understood. The metabolic hypothesis as well as the amyloid and tau hypotheses have been proposed to be associated with AD pathogenesis. In order to identify metabolome signatures from the postmortem brains of sporadic All patients and control subjects, we performed ultra performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometer (UPLC-LTQ-Orbitrap-MS). Not only our study identified new metabolome signatures but also verified previously known metabolome profiles in the brain. Statistical modeling of the analytical data and validation of the structural assignments discovered metabolic biomarkers associated with the AD pathogenesis. Interestingly, hypotaurin, myo-inositol and oxo-proline levels were markedly elevated in AD while glutamate and N-acetyl-aspartate were decreased in the postmortem brain tissue of AD patients. In addition, neurosteroid level such as cortisol was significantly increased in AD. Together, our data indicate that impaired amino acid metabolism is associated with AD pathogenesis and the altered amino acid signatures can be useful diagnostic biomarkers of All. Thus, modulation of amino acid metabolism may be a possible therapeutic approach to treat AD. English 한국뇌신경과학회 Metabolomic Analysis Identifies Alterations of Amino Acid Metabolome Signatures in the Postmortem Brain of Alzheimer's Disease Article 10.5607/en.2019.28.3.376 1 Experimental Neurobiology, v.28, no.3, pp.376 - 389 Experimental Neurobiology 28 3 376 389 N scie scopus kci ART002485985 000474466200007 2-s2.0-85069496263 Medicine, Research & Experimental Neurosciences Research & Experimental Medicine Neurosciences & Neurology Article DISCRIMINANT-ANALYSIS N-ACETYLASPARTATE OXIDATIVE STRESS MOUSE-BRAIN HPLC-MS TAURINE GLUTAMATE STRATEGY DISCOVERY DIAGNOSIS Alzheimer&apos s disease Metabolomics Liquid chromatography mass spectrometry Biomarkers amino acid metabolism