Gong, Gyeongtaek Oh, Hyun Ju Cho, Sukhyeong Kim, Seil Oh, Min-Kyu Um, Youngsoon Lee, Sun-Mi 2024-01-19T20:01:55Z 2024-01-19T20:01:55Z 2021-09-02 2019-06 0343-8651 https://pubs.kist.re.kr/handle/201004/119951 Several bioprocessing technologies, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP), have been highlighted to produce bio-based fuels and chemicals from lignocellulosic biomass. Successful CBP, an efficient and economical lignocellulosic biorefinery process compared with other processes, requires microorganisms with sufficient cellulolytic activity and biofuel/chemical-producing ability. Here, we report the complete genome of Paenibacillus sp. CAA11, a newly isolated promising microbial host for CBP-producing ethanol and organic acids from cellulose. The genome of Paenibacillus sp. CAA11 comprises one 4,888,410bp chromosome with a G + C content of 48.68% containing 4418 protein-coding genes, 102 tRNA genes, and 39 rRNA genes. The functionally active cellulase, encoded by CAA_GH5 was identified to belong to glycosyl hydrolase family 5 (GH5) and consisted of a catalytic domain and a cellulose-binding domain 3 (CBM3). When cellulolytic activity of CAA_GH5 was assayed through Congo red method by measuring the size of halo zone, the recombinant Bacillus subtilis RIK1285 expressing CAA_GH5 showed a comparable cellulolytic activity to B. subtilis RIK1285 expressing Cel5, a previously verified powerful bacterial cellulase. This study demonstrates the potential of Paenibacillus sp. CAA11 as a CBP-enabling microbe for cost-effective biofuels/chemicals production from lignocellulosic biomass. English Springer Verlag Complete Genome Sequence of Paenibacillus sp. CAA11: A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing Article 10.1007/s00284-019-01685-w 1 Current Microbiology, v.76, no.6, pp.732 - 737 Current Microbiology 76 6 732 737 N scie scopus 000467451900011 2-s2.0-85064571275 Microbiology Microbiology Article CELLULOLYTIC ABILITY PRETREATMENT