Lee, Ga-Yeon Bong, Ji-Hong Kim, Ji Yun Yoo, Gu Park, Min Kang, Min-Jung Jose, Joachim Pyun, Jae-Chul 2024-01-19T20:02:52Z 2024-01-19T20:02:52Z 2021-09-02 2019-05-09 0003-2670 https://pubs.kist.re.kr/handle/201004/120004 The autoimmune diseases systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS) are diagnosed by detection of autoantibodies against Ro and La protein autoantigens, respectively. In this work, the diagnosis of autoimmune diseases SLE and SS was demonstrated using thermophoresis of Escherichia coli with the autodisplayed autoantigens. Ro and La protein autoantigens were autodisplayed by constitutive expression together with a fluorescent protein called tdTomato in the cytosol. The binding affinity of the autodisplayed autoantigens was tested against positive and control sera by using FACS as a reference method. The factors influencing interactions between E. coli with autodisplayed autoantigens and autoantibodies in sera during thermophoresis were analyzed by measurement of cell surface charge and size before and after interaction. Finally, the thermophoretic diagnosis of autoimmune diseases SLE and SS was demonstrated using sera from patients afflicted with the respective diseases by estimating sensitivity and selectivity from ROC plots. (C) 2018 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV QUARTZ-CRYSTAL MICROBALANCE MICROSCALE THERMOPHORESIS PROTEIN ANTIBODIES PROMOTER DISPLAY ASSAY Thermophoretic diagnosis of autoimmune diseases based on Escherichia coli with autodisplayed autoantigens Article 10.1016/j.aca.2018.12.038 1 ANALYTICA CHIMICA ACTA, v.1055, pp.106 - 114 ANALYTICA CHIMICA ACTA 1055 106 114 scie scopus 000458995400013 2-s2.0-85059399084 Chemistry, Analytical Chemistry Article QUARTZ-CRYSTAL MICROBALANCE MICROSCALE THERMOPHORESIS PROTEIN ANTIBODIES PROMOTER DISPLAY ASSAY Thermophoresis Autoimmune disease Systemic lupus erythematosus Sjogren&apos s syndrome Ro protein La protein