Van, Se Young Noh, Yong Kwan Kim, Seong Who Oh, Yeon Mok Kim, Ik Hwan Park, Kwideok 2024-01-19T20:03:38Z 2024-01-19T20:03:38Z 2021-09-02 2019-05 0302-766X https://pubs.kist.re.kr/handle/201004/120044 Large expansion of human mesenchymal stem cells (MSCs) is of great interest for clinical applications. In this study, we examine the feasibility of human fibroblast-derived extracellular matrix (hFDM) as an alternative cell expansion setting. hFDM is obtained from decellularized extracellular matrix (ECM) derived from in vitro cultured human lung fibroblasts. Our study directly compares conventional platforms (tissue culture plastic (TCP), fibronectin (FN)-coated TCP) with hFDM using umbilical cord blood-derived MSCs (UCB-MSCs). Early cell morphology shows a rather rounded shape on TCP but highly elongated morphology on hFDM. Cell proliferation demonstrates that MSCs on hFDM were significantly better compared to the others in both 10 and 2% serum condition. Cell migration assay suggests that cell motility was improved and a cell migration marker CXCR4 was notably up-regulated on hFDM. MSCs differentiation into osteogenic lineage on hFDM was also very effective as examined via gene expression, von Kossa staining and alkaline phosphatase activity. In addition, as the MSCs were expanded on each substrate, transferred to 3D polymer mesh scaffolds and then cultivated for a while, the data found better cell proliferation and more CXCR4 expression with MSCs pre-conditioned on hFDM. Moreover, higher gene expression of stemness and engraftment-related markers was noticed with the hFDM group. Furthermore when UCB-MSCs expanded on TCP or hFDM were injected into emphysema (a lung disease) animal model, the results indicate that MSCs pre-conditioned on hFDM (with 2% serum) retain more advanced therapeutic efficacy on the improvement of emphysema than those on TCP. Current works demonstrate that compared to the conventional platforms, hFDM can be a promising source of cell expansion with a naturally derived biomimetic ECM microenvironment and may find some practical applications in regenerative medicine. English SPRINGER MULTI-LINEAGE DIFFERENTIATION BONE-MARROW EXTRACELLULAR-MATRIX EX-VIVO Human umbilical cord blood mesenchymal stem cells expansion via human fibroblast-derived matrix and their potentials toward regenerative application Article 10.1007/s00441-018-2971-2 1 CELL AND TISSUE RESEARCH, v.376, no.2, pp.233 - 245 CELL AND TISSUE RESEARCH 376 2 233 245 scie scopus 000464881400009 2-s2.0-85059518322 Cell Biology Cell Biology Article MULTI-LINEAGE DIFFERENTIATION BONE-MARROW EXTRACELLULAR-MATRIX EX-VIVO Extracellular matrix (ECM) Human fibroblast-derived matrix (hFDM) Umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) MSCs expansion Emphysema