Kim, Hong Nam Habbit, Nicole L. Su, Chia-Yi Choi, Nakwon Ahn, Eun Hyun Lipke, Elizabeth A. Kim, Deok-Ho 2024-01-19T20:03:45Z 2024-01-19T20:03:45Z 2021-09-02 2019-05 1616-301X https://pubs.kist.re.kr/handle/201004/120050 Tumor cell heterogeneity with distinct phenotypes, genotypes, and epigenetic states as well as the complex tumor microenvironment is major challenges for cancer diagnosis and treatment. There have been substantial advances in our knowledge of tumor biology and in the capabilities of available biological analysis tools; however, the absence of physiologically relevant in vitro testing platforms limits our ability to gain an in-depth understanding of the role of the tumor microenvironment in cancer pathology. In this review, recent advances in engineered tumor microenvironments to advance cancer research and drug discovery are presented, including tumor spheroids, microfluidic chips, paper scaffolds, hydrogel-based engineered tissues, 3D bioprinted scaffolds, and multiscale topography. Furthermore, how these technologies address the specific characteristics of the native tumor microenvironment is described. Through the comparison of these biomimetic 3D tumor models to conventional 2D culture models, the validity and physiological relevance of these platforms for fundamental in vitro studies of the tumor biology, as well as their potential use in drug screening applications, is also discussed. English WILEY-V C H VERLAG GMBH IN-VITRO MODEL 3-DIMENSIONAL CELL-CULTURE ON-A-CHIP BREAST-CANCER INTERSTITIAL FLOW EXTRACELLULAR-MATRIX MICROFLUIDIC PLATFORM GENETIC-HETEROGENEITY ANTICANCER DRUGS MIGRATION Microphysiological Systems as Enabling Tools for Modeling Complexity in the Tumor Microenvironment and Accelerating Cancer Drug Development Article 10.1002/adfm.201807553 1 ADVANCED FUNCTIONAL MATERIALS, v.29, no.22 ADVANCED FUNCTIONAL MATERIALS 29 22 scie scopus 000476566300003 2-s2.0-85062363594 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Review IN-VITRO MODEL 3-DIMENSIONAL CELL-CULTURE ON-A-CHIP BREAST-CANCER INTERSTITIAL FLOW EXTRACELLULAR-MATRIX MICROFLUIDIC PLATFORM GENETIC-HETEROGENEITY ANTICANCER DRUGS MIGRATION 3D bioprinting hydrogels microfluidics microphysiological systems paper scaffolds spheroids topography tumor microenvironments