Binh Thanh Nguyen Kang, Min-Jung 2024-01-19T20:03:55Z 2024-01-19T20:03:55Z 2021-09-02 2019-05 1420-3049 https://pubs.kist.re.kr/handle/201004/120059 Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods. The use of CE-LIF in immunoassays and enzyme assays has gained a reputation in recent years for its high detection sensitivity, short analysis time, and accurate quantification. Immunoassays are bioassay platforms that rely on binding reactions between an antigen (analyte) and a specific antibody. Enzyme assays measure enzymatic activity through quantitative analysis of substrates and products by the reaction of enzymes in purified enzyme or cell systems. These two category analyses play an important role in the context of biopharmaceutical analysis, clinical therapy, drug discovery, and diagnosis analysis. This review discusses the expanding portfolio of immune and enzyme assays using CE-LIF and focuses on the advantages and disadvantages of these methods over the ten years of existing technology since 2008. English Multidisciplinary Digital Publishing Institute (MDPI) Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays Article 10.3390/molecules24101977 1 Molecules, v.24, no.10 Molecules 24 10 Y scie scopus 000470996600135 2-s2.0-85066286793 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Review ONLINE SERS DETECTION ZONE-ELECTROPHORESIS COMPETITIVE IMMUNOASSAY MONOCLONAL-ANTIBODY MASS-SPECTROMETRY AMINO-ACIDS SENSITIVE DETERMINATION QUANTITATIVE-ANALYSIS HISTONE DEACETYLASES GEL-ELECTROPHORESIS CE-LIF immunoassay enzyme assay chip-based CE-LIF assay