Lee, Hee Ju Dhodary, Basanta Lee, Ju Yong An, Jin-Pyo Ryu, Young-Kyoung Kim, Kyoung-Shim Lee, Chul-Ho Oh, Won Keun 2024-01-19T20:04:45Z 2024-01-19T20:04:45Z 2021-09-02 2019-04-11 1420-3049 https://pubs.kist.re.kr/handle/201004/120104 Humulus japonicus is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson's disease from H. japonicus fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-O-glucoside and apigenin-7-O-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson's disease mouse model. English MDPI MONOAMINE-OXIDASE ALZHEIMERS-DISEASE L-DOPA GLYCOSIDES INHIBITION TOXICITY DEATH RATS Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It's Protective Effects against Parkinson's Disease Mouse Model Article 10.3390/molecules24071435 1 MOLECULES, v.24, no.7 MOLECULES 24 7 scie scopus 000464952900011 2-s2.0-85064707160 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article MONOAMINE-OXIDASE ALZHEIMERS-DISEASE L-DOPA GLYCOSIDES INHIBITION TOXICITY DEATH RATS Humulus japonicus HPLC-qTOF-MS in vitro MAO B assay in vivo 6-OHDA-lesioned PD animal test Parkinson&apos s Disease