Shrestha, Kshitiz Raj Jeon, Seung Hwan Jung, Ae Ryang Kim, In Gul Kim, Ga Eun Park, Yong Hyun Kim, Soo Hyun Lee, Ji Youl 2024-01-19T20:31:17Z 2024-01-19T20:31:17Z 2021-09-02 2019-04 1738-2696 https://pubs.kist.re.kr/handle/201004/120172 Background:To investigate whether human adipose-derived stem cells (hADSCs) seeded on multilayered poly (l-lactide-co--caprolactone) (PLCL) sheets improve bladder function in a rat model of detrusor smooth muscle-removed bladder.Methods:Male rats were randomly divided into 4 groups: Normal, injury (detrusor smooth muscle-removed bladder), PLCL (detrusor smooth muscle-removed bladder implanted with PLCL sheets), and PLCL+ADSC (detrusor smooth muscle-removed bladder implanted with PLCL sheets seeded with hADSCs). Four weeks after the treatment, physiological, histological, immunohistochemical, and immunoblot analyses were performed. Results:hADSCs were compatible with PLCL sheets. Further, the physiological study of PLCL+ADSC group showed significant improvement in compliance and contractility suggesting the functional improvement of the bladder. Histological, immunohistochemical and immunoblot analyses revealed the uniform distribution of hADSCs in between PLCL sheets as well as differentiation of hADSCs into smooth muscle cells (SMC) which is illustrated by the expression of SMC markers.Conclusion:hADSCs seeded on the multilayered PLCL sheets has the potential to differentiate into SMC, thus facilitating the recovery of compliance and contractility of the injured bladder. English KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC UROTHELIAL CELLS ACELLULAR MATRIX TISSUE ADIPOSE AUGMENTATION DERIVATION DELIVERY Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function Article 10.1007/s13770-019-00187-x 1 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.16, no.2, pp.201 - 212 TISSUE ENGINEERING AND REGENERATIVE MEDICINE 16 2 201 212 scie scopus kci ART002455496 000463676800009 2-s2.0-85063613587 Cell & Tissue Engineering Engineering, Biomedical Cell Biology Engineering Article UROTHELIAL CELLS ACELLULAR MATRIX TISSUE ADIPOSE AUGMENTATION DERIVATION DELIVERY Urinary bladder Smooth muscle Tissue engineering Stem cells Compliance